Szczegóły obiektu: Pathogenetic features of the nitric oxide system state in the left ventricular myocardium of the rats with experimental arterial hypertension

Instytucja dostarczająca:Akademicka Platforma Czasopism

Opis

Tytuł:
Pathogenetic features of the nitric oxide system state in the left ventricular myocardium of the rats with experimental arterial hypertension
Twórca:
Isachenko, Mariia Igorivna;
Melnikova, Olha Valeriiva
Opis:
The aim was to determine the pathogenetic features of the NO system in the myocardium of the left ventricle in the rats with endocrine-salt hypertension.Material and methods. The experiment was conducted on 20 male rats 220-290 g weight, 6–10 months old which were divided into 2 experimental groups: 1st – the control group (10 intact normotensive male Wistar rats); 2nd – 10 male Wistar rats with endocrine-salt arterial hypertension. Systolic and diastolic BP levels were measured in all the rats using a system of non-invasive arterial pressure measurement BP-2000. The objects of study in the experimental animals were blood plasma in which the nitrotyrosine level was measured, and the fragment of left ventricle, which was divided into two parts, one of which was homogenized using a Silent Crusher S homogenizer (Heidolph, Germany), in which nitrites level was determined. Concentration of immunoreactive material to NOS isoforms was detected with immunofluorescence method. The study of NOS mRNA isoforms expression in the left ventricular myocardium homogenates was carried out using a real-time polymerase chain reactionResults. In the experiment, an increase in all 3 isoforms and their mRNA was obtained. In the study, a decrease in nitrites concentration and a significant increase in nitrotyrosine levels, indicates the development of nitroso-oxidative stress.Conclusions. The development of experimental endocrine-salt hypertension in the Wistar rats leads to a stable increase in mean blood pressure by 65 % compared to control. Endocrine-salt arterial hypertension in Wistar rats is characterized by an increased mRNA content of all three isoforms of nNOS, eNOS and iNOS by 2.7, 2.8 and 5.7 times, respectively, compared to the control; increased expression of immunoreactive material to isoforms in transverse fibers by 14.3 %, 16.2 % and 18.5 %, respectively; in longitudinal fibers IRM to nNOS was higher by 8.3 %, to iNOS - by 8. 5%, but to eNOS it was lower by 7.6 %. At the same time, nitrites level decreased by 11.7 % and nitrotyrosine concentration was significantly higher, exceeding the control value by 88.5 %.
Dostępność obiektu:
Dostęp otwarty
Prawa:
Prawa autorskie (c) 2020 © The Author(s)
Data:
2020.08.31
Typ:
info:eu-repo/semantics/article;
info:eu-repo/semantics/publishedVersion
Język:
angielski
Źródło:
Journal of Education, Health and Sport; Vol. 10 No. 8 (2020); 476-489;
Journal of Education, Health and Sport; Tom 10 Nr 8 (2020); 476-489;
2391-8306
Format:
application/pdf
Wydawca:
Nicolaus Copernicus University, Toruń, Poland
Relacja: https://apcz.umk.pl/JEHS/article/view/JEHS.2020.10.08.058/26485
Temat i słowa kluczowe:
nitric oxide synthase;
NOS isoforms;
myocardium;
left ventricle;
heart;
Wistar rats;
endocrine-salt arterial hypertension;
secondary hypertension.
Identyfikator: https://apcz.umk.pl/JEHS/article/view/JEHS.2020.10.08.058
Dostawca danych:
Akademicka Platforma Czasopism

Podobne obiekty

Pathogenetic features of the nitric oxide system state in the left ventricular myocardium of the rats with experimental arterial hypertension

Twórca:Isachenko, Mariia Igorivna | Melnikova, Olha Valeriiva

Data:2020.08.31

Rodzaj zawartości:teksty

Pathogenetic features of morphodensitometric characteristics of cardiomyocytes and marker profile of the left ventricular remodeling in rats with experimental intermittent hypoxia of different duration

Twórca:Kolesnyk, Yu. M. | Isachenko, M. I.

Data:2020.09.25

Rodzaj zawartości:teksty

Experimental study of subacute oral, dermal and inhalation toxicity of Bulmoscide preparation

Data:1993

Rodzaj zawartości:pozostałe

Effects of renal nerve stimulation on intrarenal blood flow in rats with intact or inactivated NO synthases

Twórca:Walkowska, Agnieszka | Badzyńska, Bożena | Kompanowska-Jezierska, Elżbieta | Johns, EJ | Sadowski, Janusz

Data:2005.12.31 | 2005

Rodzaj zawartości:pozostałe

Biological evaluation of the aggressiveness of dust formed during the manufacture of carbon electrodes in the carbon electrode plant

Twórca:Żyłka-Włoszczyk, Maria | Szaflarska-Stojko, Ewa

Data:1989

Rodzaj zawartości:pozostałe

Аcute renal failure in patients with implanted LVAD in the early postoperative period

Twórca:Mazurenko, O. | Tarabrin, O.

Data:2023.08.29

Rodzaj zawartości:teksty

Comparative hygienic assessment of hard water impact before and after its stabilization treatment on the functional state of the central nervous system and kidneys of rats (on the example of the Olkhovska water treatment plant, Ukraine)

Data:2020.01.13

Rodzaj zawartości:teksty

Sodium nitrate affects myocardium in pregnant rats and their pups

Twórca:Sataieva, Tatiana | Zadnipryany, Igor | Zukow, Walery

Data:2018.07.31

Rodzaj zawartości:teksty

Federacja Bibliotek Cyfrowych

Szczegóły obiektu: Pathogenetic features of the nitric oxide system state in the left ventricular myocardium of the rats with experimental arterial hypertension

Opis

Podobne obiekty