Object's details: Pathogenetic features of the nitric oxide system state in the left ventricular myocardium of the rats with experimental arterial hypertension

Provider:Akademicka Platforma Czasopism

Description

Title:
Pathogenetic features of the nitric oxide system state in the left ventricular myocardium of the rats with experimental arterial hypertension
Creator:
Isachenko, Mariia Igorivna;
Melnikova, Olha Valeriiva
Description:
The aim was to determine the pathogenetic features of the NO system in the myocardium of the left ventricle in the rats with endocrine-salt hypertension.Material and methods. The experiment was conducted on 20 male rats 220-290 g weight, 6–10 months old which were divided into 2 experimental groups: 1st – the control group (10 intact normotensive male Wistar rats); 2nd – 10 male Wistar rats with endocrine-salt arterial hypertension. Systolic and diastolic BP levels were measured in all the rats using a system of non-invasive arterial pressure measurement BP-2000. The objects of study in the experimental animals were blood plasma in which the nitrotyrosine level was measured, and the fragment of left ventricle, which was divided into two parts, one of which was homogenized using a Silent Crusher S homogenizer (Heidolph, Germany), in which nitrites level was determined. Concentration of immunoreactive material to NOS isoforms was detected with immunofluorescence method. The study of NOS mRNA isoforms expression in the left ventricular myocardium homogenates was carried out using a real-time polymerase chain reactionResults. In the experiment, an increase in all 3 isoforms and their mRNA was obtained. In the study, a decrease in nitrites concentration and a significant increase in nitrotyrosine levels, indicates the development of nitroso-oxidative stress.Conclusions. The development of experimental endocrine-salt hypertension in the Wistar rats leads to a stable increase in mean blood pressure by 65 % compared to control. Endocrine-salt arterial hypertension in Wistar rats is characterized by an increased mRNA content of all three isoforms of nNOS, eNOS and iNOS by 2.7, 2.8 and 5.7 times, respectively, compared to the control; increased expression of immunoreactive material to isoforms in transverse fibers by 14.3 %, 16.2 % and 18.5 %, respectively; in longitudinal fibers IRM to nNOS was higher by 8.3 %, to iNOS - by 8. 5%, but to eNOS it was lower by 7.6 %. At the same time, nitrites level decreased by 11.7 % and nitrotyrosine concentration was significantly higher, exceeding the control value by 88.5 %.
Object availability:
free access
Rights:
Prawa autorskie (c) 2020 © The Author(s)
Date:
2020.08.31
Type:
info:eu-repo/semantics/article;
info:eu-repo/semantics/publishedVersion
Language:
English
Source:
Journal of Education, Health and Sport; Vol. 10 No. 8 (2020); 476-489;
Journal of Education, Health and Sport; Tom 10 Nr 8 (2020); 476-489;
2391-8306
Format:
application/pdf
Publisher:
Nicolaus Copernicus University, Toruń, Poland
Relation: https://apcz.umk.pl/JEHS/article/view/JEHS.2020.10.08.058/26485
Subject:
nitric oxide synthase;
NOS isoforms;
myocardium;
left ventricle;
heart;
Wistar rats;
endocrine-salt arterial hypertension;
secondary hypertension.
Identifier: https://apcz.umk.pl/JEHS/article/view/JEHS.2020.10.08.058
Data provider:
Akademicka Platforma Czasopism

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Object's details: Pathogenetic features of the nitric oxide system state in the left ventricular myocardium of the rats with experimental arterial hypertension

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