Szczegóły obiektu: Overexpression of erg20 gene encoding farnesyl pyrophosphate synthase has contrasting effects on activity of enzymes of the dolichyl and sterol branches of mevalonate pathway in Trichoderma reesei.

Instytucja dostarczająca:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Opis

Tytuł:
Overexpression of erg20 gene encoding farnesyl pyrophosphate synthase has contrasting effects on activity of enzymes of the dolichyl and sterol branches of mevalonate pathway in Trichoderma reesei.
Twórca:
Piłsyk, Sebastian;
Perlinska-Lenart, Urszula;
Gorka-Niec, Wioletta;
Graczyk, Sebastian;
Antosiewicz, Beata;
Zembek, Patrycja;
Palamarczyk, Grazyna;
Kruszewska, Joanna S.
Opis:
The mevalonate pathway is the most diverse metabolic route resulting in the biosynthesis of at least 30 000 isoprenoid compounds, many of which, such as sterols or dolichols, are indispensable for living cells. In the filamentous fungus Trichoderma of major biotechnological interest isoprenoid metabolites are also involved in the biocontrol processes giving the mevalonate pathway an additional significance. On the other hand, little is known about genes coding for enzymes of the mevalonate pathway in Trichoderma. Here, we present cloning and functional analysis of the erg20 gene from T. reesei coding for farnesyl pyrophosphate (FPP) synthase (EC 2.5.1.10), an enzyme located at the branching point of the mevalonate pathway. Expression of the gene in a thermosensitive erg20-2 mutant of Saccharomyces cerevisiae impaired in the FPP synthase activity suppressed the thermosensitive phenotype. The same gene overexpressed in T. reesei significantly enhanced FPP synthase activity and also stimulated the activity of cis-prenyltransferase, an enzyme of the dolichyl branch of the mevalonate pathway. Unexpectedly, the activity of squalene synthase from the other, sterol branch, was significantly decreased without, however, affecting ergosterol level.
Dostępność obiektu:
Dostęp otwarty
Data:
2014
Typ:
Article;
PeerReviewed
Język:
angielski
Format:
application/msword
Relacja: http://eprints.ibb.waw.pl/686 ;http://www.sciencedirect.com/science/article/pii/S0378111914005150
Temat i słowa kluczowe:
Q Science (General)
Identyfikator: http://eprints.ibb.waw.pl/686/1/ERG20.docx ;
Piłsyk, Sebastian and Perlinska-Lenart, Urszula and Gorka-Niec, Wioletta and Graczyk, Sebastian and Antosiewicz, Beata and Zembek, Patrycja and Palamarczyk, Grazyna and Kruszewska, Joanna S. (2014) Overexpression of erg20 gene encoding farnesyl pyrophosphate synthase has contrasting effects on activity of enzymes of the dolichyl and sterol branches of mevalonate pathway in Trichoderma reesei. Gene, 544 (2). pp. 114-122. (In Press)
Dostawca danych:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Podobne obiekty

Overexpression of erg20 gene encoding farnesyl pyrophosphate synthase has contrasting effects on activity of enzymes of the dolichyl and sterol branches of mevalonate pathway in Trichoderma reesei.

Data:2014

Rodzaj zawartości:pozostałe

Integration of additional copies of Trichoderma reesei gene encoding protein O-mannosyltransferase I results in a decrease of the enzyme activity and alteration of cell wall composition.

Data:2011

Rodzaj zawartości:pozostałe

Elevated activity of dolichyl phosphate mannose synthase enhances biocontrol abilities of Trichoderma atroviride.

Data:2011

Rodzaj zawartości:pozostałe

Inhibition of Dephosphorylation of Dolichyl Diphosphate Alters the Synthesis of Dolichol and Hinders Protein N-Glycosylation and Morphological Transitions in Candida albicans

Data:2019

Rodzaj zawartości:pozostałe

Yil102c-A is a Functional Homologue of the DPMII Subunit of Dolichyl Phosphate Mannose Synthase in Saccharomyces cerevisiae

Data:2020

Rodzaj zawartości:pozostałe

Dolichol phosphate mannose synthase from the pathogenic yeast Candida albicans is a multimeric enzyme.

Twórca:Juchimiuk, Mateusz | Kruszewska, Joanna S. | Palamarczyk, Grazyna

Data:2015

Rodzaj zawartości:pozostałe

Internalization of the Aspergillus nidulans AstA Transporter into Mitochondria Depends on Growth Conditions, and Affects ATP Levels and Sulfite Oxidase Activity

Twórca:Piłsyk, Sebastian | Mieczkowski, Adam | Golan, Maciej | Wawrzyniak, Agata | Kruszewska, Joanna S.

Data:2020.10.19

Rodzaj zawartości:pozostałe

KAEA (SUDPRO), a member of the ubiquitous KEOPS/EKC protein complex, regulates the arginine catabolic pathway and the expression of several other genes in Aspergillus nidulans

Data:2015.12.01

Rodzaj zawartości:pozostałe

Federacja Bibliotek Cyfrowych

Szczegóły obiektu: Overexpression of erg20 gene encoding farnesyl pyrophosphate synthase has contrasting effects on activity of enzymes of the dolichyl and sterol branches of mevalonate pathway in Trichoderma reesei.

Opis

Podobne obiekty