Opis

• Tytuł:
  Oxidative stress triggers aggregation of GFP-tagged Hsp31p, the budding yeast environmental stress response chaperone, and glyoxalase III
• Twórca:
  Natkańska, Urszula;
  Skoneczna, Adrianna;
  Skoneczny, Marek
• Opis:
  The Saccharomyces cerevisiae Hsp31p protein belongs to the ubiquitous DJ-1/ThiJ/PfpI family. The most prominent member of this family is human DJ-1; defects of this protein are associated with Parkinson's disease pathogenesis. Numerous recent findings reported by our group and others have revealed the importance of Hsp31p for survival in the post-diauxic phase of cell growth and under diverse environmental stresses. Hsp31p was shown to possess glutathione-independent glyoxalase III activity and to function as a protein chaperone, suggesting that it has multiple cellular roles. Our previous work also revealed that HSP31 gene expression was controlled by multiple stress-related transcription factors, which mediated HSP31 promoter responses to oxidative, osmotic, and thermal stresses, toxic products of glycolysis, and the diauxic shift. Nevertheless, the exact role of Hsp31p within budding yeast cells remains elusive. Here, we aimed to obtain insights into the function of Hsp31p based on its intracellular localization. We have demonstrated that the Hsp31p-GFP fusion protein is localized to the cytosol under most environmental conditions and that it becomes particulate in response to oxidative stress. However, the particles do not colocalize with other granular subcellular structures present in budding yeast cells. The observed particulate localization does not seem to be important for Hsp31p functionality. Instead, it is likely the result of oxidative damage, as the particle abundance increases when Hsp31p is nonfunctional, when the cellular oxidative stress response is affected, or when cellular maintenance systems that optimize the state of the proteome are compromised.
• Dostępność obiektu:
  Dostęp otwarty
• Data:
  2018.07
• Typ:
  Article;
  PeerReviewed
• Język:
  angielski
• Format:
  application/pdf
• Wydawca:
  Springer Netherlands
• Relacja: http://eprints.ibb.waw.pl/1591 ;https://link.springer.com/article/10.1007%2Fs12192-017-0868-8 ;doi: 10.1007/s12192-017-0868-8
• Temat i słowa kluczowe:
  QH301 Biology;
  QR Microbiology
• Identyfikator: http://eprints.ibb.waw.pl/1591/1/CSC2018_Oxidative_stress_triggers_aggregation_of_GFP-tagged_Hsp31p.pdf ;
  Natkańska, Urszula and Skoneczna, Adrianna and Skoneczny, Marek (2018) Oxidative stress triggers aggregation of GFP-tagged Hsp31p, the budding yeast environmental stress response chaperone, and glyoxalase III. Cell Stress and Chaperones, 23 (4). pp. 595-607. ISSN 1355-8145
• Dostawca danych:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
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