Description

• Tytuł:
  Relationship between therapeutic efficacy of doxorubicin-transferrin conjugate and expression of P-glycoprotein in chronic erythromyeloblastoid leukemia cells sensitive and resistant to doxorubicin
• Twórca:
  Szwed, Marzena;
  Kania, Katarzyna D.;
  Jozwiak, Zofia
• Współtwórca:
  Szwed Marzena, University of Lodz, Department of Thermobiology, Faculty of Biology and Environmental Protection,;
  Kania Katarzyna D., Institute for Medical Biology, Laboratory of Transcriptional Regulation.;
  Jozwiak Zofia, University of Lodz, Department of Thermobiology, Faculty of Biology and Environmental Protection.;
  szwedma@biol.uni.lodz.pl
• Opis:
  Background Conjugation of anti-neoplastic agents with human proteins is a strategy to diminish the toxic side effects of anthracycline antibiotics. We have developed a novel doxorubicin-transferrin (DOX-TRF) conjugate aimed to direct anticancer drugs against therapeutic targets that display altered levels of expression in malignant versus normal cells. Our previous work has shown that the cellular bio-distribution of the conjugate is dependent on a dynamic balance between influx and efflux processes. Here, we set out to investigate whether P-glycoprotein (P-gp) expression may affect DOXTRF conjugate-induced cellular drug accumulation and cytotoxicity. Results All experiments were carried out on human erythromyeloblastoid cells exhibiting P-gp over-expression (K562/DOX) and its drug sensitive parental line (K562). MTT cytotoxicity, flow cytometry, fluorescence microscopy and RT-PCR assessments revealed that the investigated conjugate (DOX-TRF) possesses a greater cytotoxic potential than free DOX. Conclusion Our data suggest that the newly developed DOXTRF conjugate is a less P-gp dependent substrate than free DOX and, consequently, may be used in a clinical setting to increase treatment efficacy in resistant human tumors.;
  Grant no. 545/ 756 of the University of Lodz, Poland.
• Dostępność obiektu:
  free access
• Prawa:
  Uznanie autorstwa 3.0 Polska;
  http://creativecommons.org/licenses/by/3.0/pl
• Data:
  2015.06.17;
  2014.11.20
• Typ:
  Article
• Język:
  English
• Format:
  application/pdf
• Wydawca:
  International Society for Cellular Oncology, IOS Press
• Relacja:
  Cell Oncology (Dordrecht);(2014) 37
• Temat i słowa kluczowe:
  Doxorubicin;
  Transferrin;
  Drug carriers;
  Multidrug resistance;
  P-glycoprotein
• Identyfikator:
  2211-3436;
  http://hdl.handle.net/11089/9915
• Dostawca danych:
  Repozytorium Uniwersytetu Łódzkiego
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