Object's details: Backbone dynamics of TFE-induced native-like fold of region 4 of Escherichia coli RNA polymerase sigma70 subunit.

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Backbone dynamics of TFE-induced native-like fold of region 4 of Escherichia coli RNA polymerase sigma70 subunit.
Creator:
Kaczka, Piotr;
Polkowska-Nowakowska, Agnieszka;
Bolewska, Krystyna;
Zhukov, Igor;
Poznański, Jarosław;
Wierzchowski, Kazimierz L
Description:
Folding of a recombinant protein rECsigma(70) (4) comprising domain 4 of E. coli RNA polymerase sigma(70) subunit, upon addition of 2,2,2-trifluoroethanol (TFE) to its aqueous solution, was monitored by heteronuclear NMR spectroscopy. The TFE-induced migration of resonance signals in a series of (15)N-HSQC spectra displayed sequence-dependent heterogeneity. A common trend of uniform upfield shift in both (1)H and (15)N dimensions, indicative of generation of helical structures, breaks down for some residues almost cooperatively at 10-15% TFE (v/v), pointing to the buildup of non-helical regions separating the initially induced helices. The preferences of residues to assume either helical or non-helical conformation are correlated with the location in the sequence rather than with their type. CSI descriptors and (15)N relaxation data obtained for the protein at 10% TFE allowed characterization of the stability of the pre-folded state of rECsigma(70) (4). By all the criteria applied, three highly populated alpha-helical regions separated by much more flexible residues forming a loop and a turn in the DNA-binding HLHTH motif were identified. The location of the secondary structure elements along the protein sequence coincides with those found in homologous proteins, and with the helix nucleation regions determined in unfolded rECsigma(70) (4) at low pH. The bimodal distribution of the (15)N relaxation parameters enabled identification of residues forming a framework of the folded protein strictly corresponding to the HLHTH motif, bracketed by unfolded terminal regions. Thus, in respect to rECsigma(70) (4) in aqueous solution TFE acts not only as a strong helix inducer, but also as a folding agent.
Object availability:
free access
Date:
2010
Type:
Article;
PeerReviewed
Language:
English
Format:
other
Relation: http://eprints.ibb.waw.pl/49
Subject:
QC Physics
Identifier: http://eprints.ibb.waw.pl/49/1/2010proteins.xps ;
Kaczka, Piotr and Polkowska-Nowakowska, Agnieszka and Bolewska, Krystyna and Zhukov, Igor and Poznański, Jarosław and Wierzchowski, Kazimierz L (2010) Backbone dynamics of TFE-induced native-like fold of region 4 of Escherichia coli RNA polymerase sigma70 subunit. Proteins, 78 (3). pp. 754-68. ISSN 1097-0134
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Similar objects

Backbone dynamics of TFE-induced native-like fold of region 4 of Escherichia coli RNA polymerase sigma70 subunit.

Date:2010

Type:other

The TFE-induced transient native-like structure of the intrinsically disordered [Formula: see text] domain of Escherichia coli RNA polymerase.

Date:2014.12

Type:other

Post-translational S-Nitrosylation Is an Endogenous Factor Fine Tuning the Properties of Human S100A1 Protein

Date:2012.11.23

Type:other

Structural Analysis of the SANT/Myb Domain of FLASH and YARP Proteins and Their Complex with the C-Terminal Fragment of NPAT by NMR Spectroscopy and Computer Simulations

Date:2020.07.24

Type:other

A Protein Data Bank Survey Reveals Shortening of Intermolecular Hydrogen Bonds in Ligand-Protein Complexes When a Halogenated Ligand Is an H-Bond Donor.

Creator:Poznański, Jarosław | Poznańska, Anna | Shugar, David

Date:2014.06.16

Type:other

Spectroscopic and thermodynamic determination of three distinct binding sites for Co(II) ions in human serum albumin.

Creator:Sokołowska, Magdalena | Wszelaka-Rylik, Małgorzata | Poznański, Jarosław | Bal, Wojciech

Date:2009

Type:other

Charged N-terminus of Influenza Fusion Peptide Facilitates Membrane Fusion

Creator:Worch, Remigiusz | Dudek, Anita | Krupa, Joanna | Szymaniec, Anna | Setny, Piotr

Date:2018.02.14

Type:other

Revisiting Mitochondrial pH with an Improved Algorithm for Calibration of the Ratiometric 5(6)-carboxy-SNARF-1 Probe Reveals Anticooperative Reaction with H+ Ions and Warrants Further Studies of Organellar pH

Date:2016.08

Type:other

Federacja Bibliotek Cyfrowych

Object's details: Backbone dynamics of TFE-induced native-like fold of region 4 of Escherichia coli RNA polymerase sigma70 subunit.

Description

Similar objects