Object's details: Charged N-terminus of Influenza Fusion Peptide Facilitates Membrane Fusion

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Charged N-terminus of Influenza Fusion Peptide Facilitates Membrane Fusion
Creator:
Worch, Remigiusz;
Dudek, Anita;
Krupa, Joanna;
Szymaniec, Anna;
Setny, Piotr
Description:
Cleavage of hemagglutinin precursor (HA0) by cellular proteases results in the formation of two subunits, HA1 and HA2. The N-terminal fragment of HA2, named a fusion peptide (HAfp), possess a charged, amine N-terminus. It has been shown that the N-terminus of HAfp stabilizes the structure of a helical hairpin observed for a 23-amino acid long peptide (HAfp1-23), whose larger activity than HAfp1-20 has been demonstrated recently. In this paper, we analyze the effect of N-terminal charge on peptide-mediated fusion efficiency and conformation changes at the membrane interface by comparison with the corresponding N-acetylated peptides of 20- and 23-amino acid lengths. We found that higher fusogenic activities of peptides with unmodified amino termini correlates with their ability to form helical hairpin structures oriented perpendicularly to the membrane plane. Molecular dynamics simulations showed that acetylated peptides adopt open and surface-bound conformation more often, which induced less disorder of the phospholipid chains, as compared to species with unmodified amino termini.
Object availability:
free access
Date:
2018.02.14
Type:
Article;
PeerReviewed
Language:
English
Format:
application/pdf
Publisher:
MDPI
Relation: http://eprints.ibb.waw.pl/1562 ;https://doi.org/10.3390/ijms19020578 ;
10.3390/ijms19020578
Subject:
QC Physics
Identifier: http://eprints.ibb.waw.pl/1562/1/ijms-19-00578.pdf ;
Worch, Remigiusz and Dudek, Anita and Krupa, Joanna and Szymaniec, Anna and Setny, Piotr (2018) Charged N-terminus of Influenza Fusion Peptide Facilitates Membrane Fusion. International Journal of Molecular Sciences, 19 (2). p. 578. ISSN 1422-0067
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: Charged N-terminus of Influenza Fusion Peptide Facilitates Membrane Fusion

Description

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