Object's details: The TFE-induced transient native-like structure of the intrinsically disordered [Formula: see text] domain of Escherichia coli RNA polymerase.

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
The TFE-induced transient native-like structure of the intrinsically disordered [Formula: see text] domain of Escherichia coli RNA polymerase.
Creator:
Kaczka, Piotr;
Winiewska, Maria;
Zhukov, Igor;
Rempola, Bozenna;
Bolewska, Krystyna;
Łoziński, Tomasz;
Ejchart, Andrzej;
Poznańska, Anna;
Wierzchowski, Kazimierz L;
Poznański, Jarosław
Description:
The transient folding of domain 4 of an E. coli RNA polymerase [Formula: see text] subunit ([Formula: see text]) induced by an increasing concentration of 2,2,2-trifluoroethanol (TFE) in an aqueous solution was monitored by means of CD and heteronuclear NMR spectroscopy. NMR data, collected at a 30 % TFE, allowed the estimation of the population of a locally folded [Formula: see text] structure (CSI descriptors) and of local backbone dynamics ((15)N relaxation). The spontaneous organization of the helical regions of the initially unfolded protein into a TFE-induced 3D structure was revealed from structural constraints deduced from (15)N- to (13)C-edited NOESY spectra. In accordance with all the applied criteria, three highly populated α-helical regions, separated by much more flexible fragments, form a transient HLHTH motif resembling those found in PDB structures resolved for homologous proteins. All the data taken together demonstrate that TFE induces a transient native-like structure in the intrinsically disordered protein.
Object availability:
free access
Date:
2014.12
Type:
Article;
PeerReviewed
Language:
English
Format:
application/msword;
application/pdf
Relation: http://eprints.ibb.waw.pl/818 ;http://link.springer.com/article/10.1007%2Fs00249-014-0987-4 ;
10.1007/s00249-014-0987-4
Subject:
Q Science (General);
QC Physics
Identifier: http://eprints.ibb.waw.pl/818/1/EBJ-140731f.doc ;http://eprints.ibb.waw.pl/818/2/EBJO-D-14-00068.pdf ;
Kaczka, Piotr and Winiewska, Maria and Zhukov, Igor and Rempola, Bozenna and Bolewska, Krystyna and Łoziński, Tomasz and Ejchart, Andrzej and Poznańska, Anna and Wierzchowski, Kazimierz L and Poznański, Jarosław (2014) The TFE-induced transient native-like structure of the intrinsically disordered [Formula: see text] domain of Escherichia coli RNA polymerase. European biophysics journal : EBJ, 43 (12). pp. 581-94. ISSN 1432-1017
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: The TFE-induced transient native-like structure of the intrinsically disordered [Formula: see text] domain of Escherichia coli RNA polymerase.

Description

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