Description

• Tytuł:
  Modeling of mRNA deadenylation rates reveal a complex relationship between mRNA deadenylation and decay
• Twórca:
  Czarnocka-Cieciura, Agnieszka;
  Poznański, Jarosław;
  Turtola, Matti Ari;
  Tomecki, Rafał;
  Krawczyk, Pawel S;
  Mroczek, Seweryn;
  Orzeł, Wiktoria;
  Saha, Upasana;
  Jensen, Torben H.;
  Dziembowski, Andrzej;
  Tudek, Agnieszka
• Opis:
  Complete cytoplasmic polyadenosine tail (polyA-tail) deadenylation is thought to be essential for initiating mRNA decapping and subsequent degradation. To investigate this prevalent model, we conducted direct RNA sequencing of S. cerevisiae mRNAs derived from chase experiments under steady-state and stress condition. Subsequently, we developed a numerical model based on a modified gamma distribution function, which estimated the transcriptomic deadenylation rate at 10 A/min. A simplified independent method, based on the delineation of quantile polyA-tail values, showed a correlation between the decay and deadenylation rates of individual mRNAs, which appeared consistent within functional transcript groups and associated with codon optimality. Notably, these rates varied during the stress response. Detailed analysis of ribosomal protein-coding mRNAs (RPG mRNAs), constituting 40% of the transcriptome, singled out this transcript group. While deadenylation and decay of RPG mRNAs accelerated under heat stress, their degradation could proceed even when deadenylation was blocked, depending entirely on ongoing nuclear export. Our findings support the general primary function of deadenylation in dictating the onset of decapping, while also demonstrating complex relations between these processes.
• Dostępność obiektu:
  free access
• Data:
  2024.10.11
• Typ:
  Article;
  PeerReviewed
• Język:
  English
• Format:
  application/pdf
• Wydawca:
  Springer
• Relacja: http://eprints.ibb.waw.pl/2476 ;https://www.embopress.org/doi/full/10.1038/s44318-024-00258-3
• Temat i słowa kluczowe:
  Q Science (General);
  QR Microbiology
• Identyfikator: http://eprints.ibb.waw.pl/2476/1/czarnocka-cieciura-et-al-2024-modeling-of-mrna-deadenylation-rates-reveal-a-complex-relationship-between-mrna.pdf ;
  Czarnocka-Cieciura, Agnieszka and Poznański, Jarosław and Turtola, Matti Ari and Tomecki, Rafał and Krawczyk, Pawel S and Mroczek, Seweryn and Orzeł, Wiktoria and Saha, Upasana and Jensen, Torben H. and Dziembowski, Andrzej and Tudek, Agnieszka (2024) Modeling of mRNA deadenylation rates reveal a complex relationship between mRNA deadenylation and decay. EMBO Journal .
• Dostawca danych:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
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