Description

• Title:
  Novel broad-host-range vehicles for cloning and shuffling of gene cassettes
• Creator:
  Bartosik, Aneta A;
  Markowska, Aleksandra;
  Szarlak, Jolanta;
  Kulinska, Anna;
  Jagura-Burdzy, Grazyna
• Description:
  Novel vectors for cloning and shuffling of gene cassettes based on minireplicon of broadhost- range RA3 plasmid from IncU incompatibility group were constructed. A series of minireplicon variants were prepared with copy number ranging from low (1-2 copies per chromosome), medium (10-15 copies per chromosome) to high copy number (80-90 copies per chromosome). The new cloning vectors are relatively small in size (4.5-5.4 kb) and carry various resistance determinants: kanamycin (KmR), tetracycline (TcR) or chloramphenicol (CmR). The vectors were engineered to facilitate cloning and shuffling of the functional modules with or without transcriptional terminators. Using the described strategy, a bank of functional modules, ready for exchange, has been initiated.
• Object availability:
  free access
• Date:
  2012
• Type:
  Article;
  PeerReviewed
• Language:
  English
• Format:
  application/pdf
• Publisher:
  Elsevier B.V.
• Relation: http://eprints.ibb.waw.pl/220 ;.com/locate/jmicmeth ;doi:10.1016/j.mimet.2011.10.011
• Subject:
  Q Science (General);
  QR Microbiology
• Identifier: http://eprints.ibb.waw.pl/220/1/MIMET-S-11-00737.pdf ;
  Bartosik, Aneta A and Markowska, Aleksandra and Szarlak, Jolanta and Kulinska, Anna and Jagura-Burdzy, Grazyna (2012) Novel broad-host-range vehicles for cloning and shuffling of gene cassettes. Journal of Microbiological Methods, 88 (1). pp. 53-62.
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  seek permission
• Type:
  other