Description

• Title:
  Strand specificity of ribonucleotide excision repair in Escherichia coli
• Creator:
  Łazowski, Krystian;
  Faraz, Mahmood;
  Vaisman, Alexandra;
  Ashton, Nicholas W;
  Jonczyk, Piotr;
  Clausen, Anders R;
  Fijalkowska, Iwona J.;
  Woodgate, Roger;
  Makiela-Dzbenska, Karolina
• Description:
  In Escherichia coli, replication of both strands of genomic DNA is carried out by a single replicase—DNA polymerase III holoenzyme (pol III HE). However, in certain genetic backgrounds, the low-fidelity TLS polymerase, DNA polymerase V (pol V) gains access to undamaged genomic DNA where it promotes elevated levels of spontaneous mutagenesis preferentially on the lagging strand. We employed active site mutants of pol III (pol IIIα_S759N) and pol V (pol V_Y11A) to analyze ribonucleotide incorporation and removal from the E. coli chromosome on a genome-wide scale under conditions of normal replication, as well as SOS induction. Using a variety of methods tuned to the specific properties of these polymerases (analysis of lacI mutational spectra, lacZ reversion assay, HydEn-seq, alkaline gel electrophoresis), we present evidence that repair of ribonucleotides from both DNA strands in E. coli is unequal. While RNase HII plays a primary role in leading-strand Ribonucleotide Excision Repair (RER), the lagging strand is subject to other repair systems (RNase HI and under conditions of SOS activation also Nucleotide Excision Repair). Importantly, we suggest that RNase HI activity can also influence the repair of single ribonucleotides incorporated by the replicase pol III HE into the lagging strand.
• Object availability:
  free access
• Date:
  2023.02.10
• Type:
  Article;
  PeerReviewed
• Language:
  English
• Format:
  application/pdf;
  other
• Publisher:
  Oxford University Press
• Relation: http://eprints.ibb.waw.pl/2249 ;https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkad038/7033787 ;
  10.1093/nar/gkad038
• Subject:
  Q Science (General);
  QR Microbiology
• Identifier: http://eprints.ibb.waw.pl/2249/1/gkad038.pdf ;http://eprints.ibb.waw.pl/2249/2/bibliography.txt ;
  Łazowski, Krystian and Faraz, Mahmood and Vaisman, Alexandra and Ashton, Nicholas W and Jonczyk, Piotr and Clausen, Anders R and Fijalkowska, Iwona J. and Woodgate, Roger and Makiela-Dzbenska, Karolina (2023) Strand specificity of ribonucleotide excision repair in Escherichia coli. Nucleic Acids Research . gkad038. ISSN 1362-4962
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  seek permission
• Type:
  other