Description

• Title:
  Impairment of the non-catalytic subunit Dpb2 of DNA Pol ɛ results in increased involvement of Pol δ on the leading strand
• Creator:
  Dmowski, Michal;
  Makiela-Dzbenska, Karolina;
  Sharma, Sushma;
  Chabes, Andrei;
  Fijalkowska, Iwona J.
• Description:
  The generally accepted model assumes that leading strand synthesis is performed by Pol ε, while lagging-strand synthesis is catalyzed by Pol δ. Pol ε has been shown to target the leading strand by interacting with the CMG helicase [Cdc45 Mcm2–7 GINS(Psf1–3, Sld5)]. Proper functioning of the CMG-Pol ε, the helicase-polymerase complex is essential for its progression and the fidelity of DNA replication. Dpb2p, the essential non-catalytic subunit of Pol ε plays a key role in maintaining the correct architecture of the replisome by acting as a link between Pol ε and the CMG complex. Using a temperature-sensitive dpb2–100 mutant previously isolated in our laboratory, and a genetic system which takes advantage of a distinct mutational signature of the Pol δ-L612M variant which allows detection of the involvement of Pol δ in the replication of particular DNA strands we show that in yeast cells with an impaired Dpb2 subunit, the contribution of Pol δ to the replication of the leading strand is significantly increased.
• Object availability:
  free access
• Date:
  2023.07.07
• Type:
  Article;
  PeerReviewed
• Language:
  English
• Format:
  application/pdf;
  other
• Publisher:
  Elsevier
• Relation: http://eprints.ibb.waw.pl/2537 ;https://doi.org/10.1016/j.dnarep.2023.103541 ;doi.org/10.1016/j.dnarep.2023.103541
• Subject:
  Q Science (General);
  QH301 Biology;
  QR Microbiology
• Identifier: http://eprints.ibb.waw.pl/2537/1/1-s2.0-S1568786423000952-main.pdf ;http://eprints.ibb.waw.pl/2537/2/1-s2.0-S1568786423000952-mmc1.pdf ;http://eprints.ibb.waw.pl/2537/7/bibliography.txt ;
  Dmowski, Michal and Makiela-Dzbenska, Karolina and Sharma, Sushma and Chabes, Andrei and Fijalkowska, Iwona J. (2023) Impairment of the non-catalytic subunit Dpb2 of DNA Pol ɛ results in increased involvement of Pol δ on the leading strand. DNA Repair, 129 . p. 103541. ISSN 1568-7864
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  seek permission
• Type:
  other