Opis

• Tytuł:
  Specific Features of RNA Polymerases I and III: Structure and Assembly
• Creator:
  Turowski, Tomasz W.;
  Boguta, Magdalena
• Description:
  RNA polymerase I (RNAPI) and RNAPIII are multi-heterogenic protein complexes that specialize in the transcription of highly abundant non-coding RNAs, such as ribosomal RNA (rRNA) and transfer RNA (tRNA). In terms of subunit number and structure, RNAPI and RNAPIII are more complex than RNAPII that synthesizes thousands of different mRNAs. Specific subunits of the yeast RNAPI and RNAPIII form associated subcomplexes that are related to parts of the RNAPII initiation factors. Prior to their delivery to the nucleus where they function, RNAP complexes are assembled at least partially in the cytoplasm. Yeast RNAPI and RNAPIII share heterodimer Rpc40-Rpc19, a functional equivalent to the αα homodimer which initiates assembly of prokaryotic RNAP. In the process of yeast RNAPI and RNAPIII biogenesis, Rpc40 and Rpc19 form the assembly platform together with two small, bona fide eukaryotic subunits, Rpb10 and Rpb12. We propose that this assembly platform is co-translationally seeded while the Rpb10 subunit is synthesized by cytoplasmic ribosome machinery. The translation of Rpb10 is stimulated by Rbs1 protein, which binds to the 3′-untranslated region of RPB10 mRNA and hypothetically brings together Rpc19 and Rpc40 subunits to form the αα-like heterodimer. We suggest that such a co-translational mechanism is involved in the assembly of RNAPI and RNAPIII complexes.
• Object availability:
  Dostęp otwarty
• Date:
  2021
• Type:
  Article;
  PeerReviewed
• Language:
  angielski
• Format:
  application/pdf
• Relation: http://eprints.ibb.waw.pl/2048 ;http://doi.org/10.3389/fmolb.2021.680090 ;doi:10.3389/fmolb.2021.680090
• Subject:
  Q Science (General);
  QR Microbiology
• Identifier: http://eprints.ibb.waw.pl/2048/1/fmolb-08-680090.pdf ;
  Turowski, Tomasz W. and Boguta, Magdalena (2021) Specific Features of RNA Polymerases I and III: Structure and Assembly. Frontiers in Molecular Biosciences, 8 . ISSN 2296-889X
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  poproś o pozwolenie
• Type:
  pozostałe