Szczegóły obiektu: Identification of pathogenic microbial cells and spores by electrochemical detection on a biochip

Instytucja dostarczająca:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Opis

Tytuł:
Identification of pathogenic microbial cells and spores by electrochemical detection on a biochip
Twórca:
Gabig-Cimińska, Magdalena;
Andresen, Heiko;
Albers, Joerg;
Hintsche, Rainer;
Enfors, Sven-Olof
Opis:
BACKGROUND: Bacillus cereus constitutes a significant cause of acute food poisoning in humans. Despite the recent development of different detection methods, new effective control measures and better diagnostic tools are required for quick and reliable detection of pathogenic micro-organisms. Thus, the objective of this study was to determine a simple method for rapid identification of enterotoxic Bacillus strains. Here, a special attention is given to an electrochemical biosensor since it meets the requirements of minimal size, lower costs and decreased power consumption. RESULTS: A bead-based sandwich hybridization system was employed in conjugation with electric chips for detection of vegetative cells and spores of Bacillus strains based on their toxin-encoding genes. The system consists of a silicon chip based potentiometric cell, and utilizes paramagnetic beads as solid carriers of the DNA probes. The specific signals from 20 amol of bacterial cell or spore DNA were achieved in less than 4 h. The method was also successful when applied directly to unpurified spore and cell extract samples. The assay for the haemolytic enterotoxin genes resulted in reproducible signals from B. cereus and B. thuringiensis while haemolysin-negative B. subtilis strain did not yield any signal. CONCLUSIONS: The sensitivity, convenience and specificity of the system have shown its potential. In this respect an electrochemical detection on a chip enabling a fast characterization and monitoring of pathogens in food is of interest. This system can offer a contribution in the rapid identification of bacteria based on the presence of specific genes without preceding nucleic acid amplification.
Dostępność obiektu:
Dostęp otwarty
Data:
2004
Typ:
Article;
PeerReviewed
Język:
angielski
Format:
application/pdf
Publisher:
BioMed Central Ltd
Relation: http://eprints.ibb.waw.pl/1072
Subject:
QH301 Biology;
QH426 Genetics;
QR Microbiology
Identifier: http://eprints.ibb.waw.pl/1072/1/1475-2859-3-2.pdf ;
Gabig-Cimińska, Magdalena and Andresen, Heiko and Albers, Joerg and Hintsche, Rainer and Enfors, Sven-Olof (2004) Identification of pathogenic microbial cells and spores by electrochemical detection on a biochip. Microbial Cell Factories, 3 . ISSN 1475-2859
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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