Opis

• Tytuł:
  ANALYSIS OF THE UTERINE MICROBIOME IN COWS WITH RETENTIO SECUNDINARUM AND THE DYNAMICS OF ITS CHANGES DURING THERAPY
• Twórca:
  Turek, J;
  Garbowicz , M
• Opis:
  Retentio secundinarum (RS) in cows is defined as the failure to expel the placenta 24 hoursafter calving. This complication is the cause of significant economic losses due toreproductive disorders including: uterine inflammation, prolonged postpartum downtime,reduced mating rates, prolonged inter-partum period and the development of secondarymetabolic diseases. These losses add to the cost of treating the animals. The microorganismsmost commonly found in RS cases were Escherichia coli, Fusobacterium necrophorum,Clostridium spp. and Trueperella pyogenes, the presence of which in these cases justified thedomaic and general use of antibiotics, including third-generation cephalosporins. Theirwidespread use in the current social context is unjustified. Other antibiotics have beensuggested for the treatment of RS. There is now a growing interest in other alternativetreatments. Nowadays, precise genetic tools are available to assess the type ofmicroorganisms. The aim of this study was to determine the uterine microbiome of cows withRS. Metagenomic analysis of the popuation of batteries and archaeons was carried out on thebasis of the hypo-ergodic V3-V4 region of the S rRNA gene. Specific primer sequences 34IFand 785R (16S analysis) were used to amplify the selected region and prepare the library.PCR was performed using Q5 Hot Start High-Fidelity 2X Master Mix, according to themanufacturer’s recommendations. Sequencing was performed on a MiSeq, paired-end (PE),2×300 nt, using Illumina’s v3 kit. Automatic pre-analysis of the data was performed on aMiseq sequencer using Miseq Reporter (MSR) v 2.6 software. The analysis consisted of twosteps: (1) automatic demultiplexing of samples and (2) generation of fastq files containingraw reads. Based on the evaluation of the preliminary results of the NGS sequencing analysis,a predominance of the bacteria Fusobacterium necrophorum, Coxiella burnetii, Esscherichiacoli, Streprococcus pluranimalium and Clostridium perfringens was observed in the testspecimens immediately after placenta detachment. It is planned to continue the study toconfirm the composition of the uterine microbiome in a larger number of animals in order todevelop a microbiome typical of RS cases and also to observe it after antibiotic and eubiotictherapy.
• Dostępność obiektu:
  Dostęp otwarty
• Prawa:
  Prawa autorskie (c) 2023 Turek J, Garbowicz M;
  https://creativecommons.org/licenses/by-nd/4.0
• Data:
  2023.07.09
• Typ:
  info:eu-repo/semantics/article;
  info:eu-repo/semantics/publishedVersion
• Język:
  angielski
• Źródło:
  Translational Research in Veterinary Science; Vol. 6 No. 1 (2023); 14;
  Translational Research In Veterinary Science; Tom 6 Nr 1 (2023); 14;
  2657-4489
• Format:
  application/pdf
• Wydawca:
  Nicolaus Copernicus University in Toruń
• Relacja: https://apcz.umk.pl/TRVS/article/view/63748/43282
• Temat i słowa kluczowe:
  Retentio secundinarum;
  uterus;
  microbiome;
  cows
• Identyfikator: https://apcz.umk.pl/TRVS/article/view/63748
• Dostawca danych:
  Akademicka Platforma Czasopism
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