Opis

• Tytuł:
  Mycobacterium bovis BCG increase the selected determinants of monocyte/macrophage activity, which were diminished in response to gastric pathogen Helicobacter pylori
• Twórca:
  Gonciarz, Weronika;
  Chyb, Maciej;
  Chmiela, Magdalena
• Współtwórca:
  Department of Immunology and Infectious Biology, Faculty of Biology and Environmental Protection, University of Lodz, 12/16 Banacha St., 90‑237 Lodz, Poland.;
  Department of Molecular Microbiology, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.;
  Bio-Med-Chem Doctoral School of the University of Lodz and Lodz Institutes of the Polish Academy of Sciences, Lodz, Poland.
• Opis:
  High antibiotic resistance of gastric pathogen Helicobacter pylori (Hp) and the ability to escape the host immune response prompt searching for therapeutic immunomodulators. Bacillus Calmette–Guerin (BCG) vaccine with Mycobacterium bovis (Mb) is a candidate for modulation the activity of immunocompetent cells, and onco-BCG formulation was successfully used in immunotherapy of bladder cancer. We determined the influence of onco-BCG on the phagocytic capacity of human THP-1 monocyte/macrophage cells, using the model of Escherichia coli bioparticles and Hp fluorescently labeled. Deposition of cell integrins CD11b, CD11d, CD18, membrane/soluble lipopolysaccharide (LPS) receptors, CD14 and sCD14, respectively, and the production of macrophage chemotactic protein (MCP)-1 were determined. Furthermore, a global DNA methylation, was also assessed. Human THP-1 monocytes/macrophages (TIB 202) primed or primed and restimulated with onco-BCG or Hp, were used for assessment of phagocytosis towards E. coli or Hp, surface (immunostaining) or soluble activity determinants, and global DNA methylation (ELISA). THP-1 monocytes/macrophages primed/restimulated with BCG showed increased phagocytosis capacity towards E. coli fluorescent particles, elevated expression of CD11b, CD11d, CD18, CD14, sCD14, increased MCP-1 secretion and DNA methylation. Preliminary results indicate that BCG mycobacteria may also induce the phagocytosis of H. pylori by THP-1 monocytes. Priming or priming and restimulation of monocytes/macrophages with BCG resulted in an increased activity of these cells, which was negatively modulated by Hp.;
  This research was financially supported by University of Lodz, Grant Number 15/GNZPA/2022 (B2111001000027.07). Development of chitozan biopolimer with Mycobacterium bovis BCG-onko vaccine mycobacteria, with immunomodulatory properties, to improve immune response towards Helicobacter pylori and Student Research Grants financed by University of Lodz.
• Dostępność obiektu:
  Dostęp otwarty
• Prawa:
  Uznanie autorstwa 4.0 Międzynarodowe;
  http://creativecommons.org/licenses/by/4.0
• Data:
  2023.05.09;
  2023
• Typ:
  Article
• Język:
  angielski
• Format:
  application/pdf
• Wydawca:
  Nature Portfolio
• Relacja:
  Scientific Reports;3107;
  13
• Temat i słowa kluczowe:
  Monocytes and macrophages;
  Bacterial host response
• Identyfikator:
  Gonciarz Weronika, Chyb Maciej, Mikołajczyk-Chmiela Magdalena: Mycobacterium bovis BCG increase the selected determinants of monocyte/ macrophage activity, which were diminished in response to gastric pathogen Helicobacter pylori, Scientific Reports, Nature Publishing Group, vol. 13, 2023, Numer artykułu: 3107, s. 1-14, DOI:10.1038/s41598-023-30250-6;
  2045-2322;
  http://hdl.handle.net/11089/46983 ;
  10.1038/s41598-023-30250-6
• Dostawca danych:
  Repozytorium Uniwersytetu Łódzkiego
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