Opis

• Tytuł:
  Impairment of the non-catalytic subunit Dpb2 of DNA Pol ε results in increased involvement of Pol δ on the leading strand
• Twórca:
  Dmowski, Michal;
  Makiela-Dzbenska, Karolina;
  Sharma, Sushma;
  Chabes, Andrei;
  Fijalkowska, Iwona J.
• Opis:
  The generally accepted model assumes that leading strand synthesis is performed by Pol ε, while lagging-strand synthesis is catalyzed by Pol δ. Pol ε has been shown to target the leading strand by interacting with the CMG helicase [Cdc45 Mcm2–7 GINS(Psf1–3, Sld5)]. Proper functioning of the CMG-Pol ε, the helicase-polymerase complex is essential for its progression and the fidelity of DNA replication. Dpb2p, the essential non-catalytic subunit of Pol ε plays a key role in maintaining the correct architecture of the replisome by acting as a link between Pol ε and the CMG complex. Using a temperature-sensitive dpb2–100 mutant previously isolated in our laboratory, and a genetic system which takes advantage of a distinct mutational signature of the Pol δ-L612M variant which allows detection of the involvement of Pol δ in the replication of particular DNA strands we show that in yeast cells with an impaired Dpb2 subunit, the contribution of Pol δ to the replication of the leading strand is significantly increased.
• Dostępność obiektu:
  Dostęp otwarty
• Data:
  2023.07.07
• Typ:
  Article;
  PeerReviewed
• Język:
  angielski
• Format:
  application/pdf;
  other
• Wydawca:
  Elsevier
• Relacja: http://eprints.ibb.waw.pl/2538 ;https://doi.org/10.1016/j.dnarep.2023.103541 ;doi.org/10.1016/j.dnarep.2023.103541
• Temat i słowa kluczowe:
  Q Science (General);
  QH301 Biology;
  QR Microbiology
• Identyfikator: http://eprints.ibb.waw.pl/2538/1/1-s2.0-S1568786423000952-main.pdf ;http://eprints.ibb.waw.pl/2538/2/1-s2.0-S1568786423000952-mmc1.pdf ;http://eprints.ibb.waw.pl/2538/7/bibliography.txt ;
  Dmowski, Michal and Makiela-Dzbenska, Karolina and Sharma, Sushma and Chabes, Andrei and Fijalkowska, Iwona J. (2023) Impairment of the non-catalytic subunit Dpb2 of DNA Pol ε results in increased involvement of Pol δ on the leading strand. DNA Repair, 129 . p. 103541. ISSN 1568-7864
• Dostawca danych:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
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