Szczegóły obiektu: Revisiting Mitochondrial pH with an Improved Algorithm for Calibration of the Ratiometric 5(6)-carboxy-SNARF-1 Probe Reveals Anticooperative Reaction with H+ Ions and Warrants Further Studies of Organellar pH

Instytucja dostarczająca:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Opis

Tytuł:
Revisiting Mitochondrial pH with an Improved Algorithm for Calibration of the Ratiometric 5(6)-carboxy-SNARF-1 Probe Reveals Anticooperative Reaction with H+ Ions and Warrants Further Studies of Organellar pH
Twórca:
Zurawik, TM;
Pomorski, Adam;
Belczyk-Ciesielska, Agnieszka;
Goch, Grażyna;
Niedźwiecka, Katarzyna;
Kucharczyk, Roza;
Krezel, Artur;
Bal, Wojciech
Opis:
Fluorescence measurements of pH and other analytes in the cell rely on accurate calibrations, but these have routinely used algorithms that inadequately describe the properties of indicators. Here, we have established a more accurate method for calibrating and analyzing data obtained using the ratiometric probe 5(6)-carboxy-SNARF-1. We tested the implications of novel approach to measurements of pH in yeast mitochondria, a compartment containing a small number of free H+ ions. Our findings demonstrate that 5(6)-carboxy-SNARF- 1 interacts with H+ ions inside the mitochondria in an anticooperative manner (Hill coefficient n of 0.5) and the apparent pH inside the mitochondria is ~0.5 unit lower than had been generally assumed. This result, at odds with the current consensus on the mechanism of energy generation in the mitochondria, is in better agreement with theoretical considerations and warrants further studies of organellar pH.
Dostępność obiektu:
Dostęp otwarty
Data:
2016.08
Typ:
Article;
PeerReviewed
Język:
angielski
Format:
application/pdf
Relacja: http://eprints.ibb.waw.pl/1309 ;https://www.ncbi.nlm.nih.gov/pubmed/27557123
Temat i słowa kluczowe:
QC Physics;
QD Chemistry
Identyfikator: http://eprints.ibb.waw.pl/1309/1/Zurawik-PLOSone_2016.PDF ;
Zurawik, TM and Pomorski, Adam and Belczyk-Ciesielska, Agnieszka and Goch, Grażyna and Niedźwiecka, Katarzyna and Kucharczyk, Roza and Krezel, Artur and Bal, Wojciech (2016) Revisiting Mitochondrial pH with an Improved Algorithm for Calibration of the Ratiometric 5(6)-carboxy-SNARF-1 Probe Reveals Anticooperative Reaction with H+ Ions and Warrants Further Studies of Organellar pH. PloS one, 11 (8). ISSN 1932-6203
Dostawca danych:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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