Opis

• Tytuł:
  Functional gene expression profile underlying methotrexate-induced senescence in human colon cancer cells.
• Twórca:
  Dabrowska, Magdalena;
  Skoneczny, Marek;
  Rode, Wojciech
• Opis:
  Cellular functions accompanying establishment of premature senescence in methotrexate-treated human colon cancer C85 cells are deciphered in the present study from validated competitive expression microarray data, analyzed with the use of Ingenuity Pathways Analysis (IPA) software. The nitrosative/oxidative stress, inferred from upregulated expression of inducible nitric oxide synthase (iNOS) and mitochondrial dysfunction-associated genes, including monoamine oxidases MAOA and MAOB, β-amyloid precursor protein (APP) and presenilin 1 (PSEN1), is identified as the main determinant of signaling pathways operating during senescence establishment. Activation of p53-signaling pathway is found associated with both apoptotic and autophagic components contributing to this process. Activation of nuclear factor κB (NF-κB), resulting from interferon γ (IFNγ), integrin, interleukin 1β (IL-1β), IL-4, IL-13, IL-22, Toll-like receptors (TLRs) 1, 2 and 3, growth factors and tumor necrosis factor (TNF) superfamily members signaling, is found to underpin inflammatory properties of senescent C85 cells. Upregulation of p21-activated kinases (PAK2 and PAK6), several Rho molecules and myosin regulatory light chains MYL12A and MYL12B, indicates acquisition of motility by those cells. Mitogen-activated protein kinase p38 MAPK β, extracellular signal-regulated kinases ERK2 and ERK5, protein kinase B AKT1, as well as calcium, are identified as factors coordinating signaling pathways in senescent C85 cells.
• Dostępność obiektu:
  Dostęp otwarty
• Data:
  2011.10
• Typ:
  Article;
  PeerReviewed
• Relacja: http://eprints.ibb.waw.pl/187
• Temat i słowa kluczowe:
  Q Science (General)
• Identyfikator:
  Dabrowska, Magdalena and Skoneczny, Marek and Rode, Wojciech (2011) Functional gene expression profile underlying methotrexate-induced senescence in human colon cancer cells. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 32 (5). pp. 965-76. ISSN 1423-0380
• Dostawca danych:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
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