Opis

• Tytuł:
  Mechanism study of alachlor biodegradation by Paecilomycesmarquandii with proteomic and metabolomic methods
• Twórca:
  Szewczyk, Rafał;
  Soboń, Adrian;
  Słaba, Mirosława;
  Długoński, Jerzy
• Współtwórca:
  Faculty of Biology and Environmental Protection, University of Łodz,
• Opis:
  Alachlor is an herbicide that is widely used worldwide to protect plant crops against broadleaf weedsand annual grasses. However, due to its endocrine-disrupting activity, its application had been bannedin the European Union. As described in our earlier work, Paecilomyces marquandii is a microscopic funguscapable of alachlor removal by N-acetyl oxidation. Our current work uses proteomics and metabolomicsto gain a better understanding of alachlor biodegradation by the microscopic fungus P. marquandii.The data revealed that the addition of alachlor reduced the culture growth and glucose consump-tion rates. Moreover, the rates of glycolysis and the tricarboxylic acids (TCA) cycle increased duringthe initial stage of growth, and there was a shift toward the formation of supplementary materials(UDP-glucose/galactose) and reactive oxygen species (ROS) scavengers (ascorbate). Proteomic analy-sis revealed that the presence of xenobiotics resulted in a strong upregulation of enzymes related toenergy, sugar metabolism and ROS production. However, the unique overexpression of cyanide hydratasein alachlor-containing cultures may implicate this enzyme as the key protein involved in the alachlorbiodegradation pathway. The characterization of P. marquandii-mediated alachlor removal in terms of cellstructure and function provides a deeper insight into the strategies of microorganisms toward xenobioticbiodegradation.;
  This study was supported by the grant of the National Science Centre, Poland (Project No. UMO-2011/01/B/NZ9/02898).
• Dostępność obiektu:
  Dostęp otwarty
• Prawa:
  Uznanie autorstwa-Użycie niekomercyjne-Bez utworów zależnych 3.0 Polska;
  http://creativecommons.org/licenses/by-nc-nd/3.0/pl
• Data:
  2015.06.15;
  2015.03.03
• Typ:
  Article
• Język:
  angielski
• Format:
  application/pdf
• Wydawca:
  Elsevier Science Limited
• Relacja:
  Journal of Hazardous Materials;Volume 291, 30 June 2015
• Temat i słowa kluczowe:
  fungi;
  alachlor;
  biodegradation;
  metabolomics;
  proteomics;
  lc-ms/ms
• Identyfikator:
  1873-3336;
  http://hdl.handle.net/11089/9735 ;
  10.1016/j.jhazmat.2015.02.063
• Dostawca danych:
  Repozytorium Uniwersytetu Łódzkiego
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