Opis

• Tytuł:
  Polyphenol extract from evening primrose pomace alleviates experimental colitis after intracolonic and oral administration in mice
• Twórca:
  Sałaga, M.;
  Lewandowska, U.;
  Sosnowska, D.;
  Zakrzewski, P. K.;
  Cygankiewicz, A. I.;
  Piechota-Polańczyk, A.;
  Sobczak, M.;
  Mosinska, P.;
  Chen, Chunqiu;
  Krajewska, W. M.;
  Fichna, J.
• Współtwórca:
  Medical University of Lodz, Faculty of Medicine;
  Technical University of Lodz, Department of Biotechnology and Food Sciences;
  University of Lodz, Faculty of Biology and Environmental Protection;
  Tongji University, School of Medicine;
  jakub.fichna@umed.lodz.pl
• Opis:
  Oenothera paradoxa (EP) preparations are commonly used in folk medicine to treat skin diseases, neuralgia, and gastrointestinal (GI) disorders. Several reports suggested that EP preparations exhibit potent anti-inflammatory and antioxidant activities both in vitro and in vivo. Here, we aimed to characterize the action of EP pomace polyphenol extract in mouse model of colitis. We analyzed the composition of EP pomace polyphenol extract using reversed phase HPLC system and ultra-performance liquid chromatography (UPLC) system coupled with a quadrupole-time of flight (Q-TOF) MS instrument. Then, we used a well-established animal model of 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis to determine the anti-inflammatory action of EP pomace polyphenol extract. We also investigated the effect of the EP pomace polyphenol extract on pro-inflammatory (IL-1β and TNF-α) cytokine mRNA levels and hydrogen peroxide concentration in the inflamed colon. Administration of EP pomace polyphenol extract significantly improved macroscopic and microscopic damage scores, as well as myeloperoxidase (MPO) activity in TNBS-treated mice. The anti-inflammatory effect of the extract was observed after intracolonic and oral administration and was dose-dependent. Significant reduction of tissue hydrogen peroxide level after treatment with EP pomace polyphenol extract suggests that its therapeutic effect is a result of free radical scavenging. This novel finding indicates that the application of the EP pomace polyphenol extract in patients with inflammatory bowel diseases (IBDs) may become an attractive supplementary treatment for conventional anti-inflammatory therapy.;
  The authors wish to thank Dr. Alicja Z. Kucharska from the Wroclaw University of Environmental and Life Sciences for determining the composition of polyphenolic compounds by UPLC-QTOF-MS. This study was supported by the bilateral cooperation between Poland and China, the Iuventus Plus program of the Polish Ministry of Science and Higher Education (0107/IP1/2013/72 to JF), and Medical University of Lodz (502-03/1-156-02/502-14-140 to M Sałaga and 503/ 1-156-04/503-01).
• Dostępność obiektu:
  Dostęp otwarty
• Prawa:
  Uznanie autorstwa 3.0 Polska;
  http://creativecommons.org/licenses/by/3.0/pl
• Data:
  2016.04.01;
  2014
• Typ:
  Article
• Język:
  angielski
• Format:
  application/pdf
• Wydawca:
  Springer Berlin Heidelberg
• Relacja:
  Naunyn-Schmiedeberg's Archives of Pharmacology;11;
  387
• Temat i słowa kluczowe:
  Evening primrose;
  Experimental colitis;
  Inflammatory bowel diseases
• Identyfikator:
  0028-1298;
  http://hdl.handle.net/11089/17652 ;
  1432-1912;
  10.1007/s00210-014-1025-x
• Dostawca danych:
  Repozytorium Uniwersytetu Łódzkiego
• Czy mogę z tego obiektu skorzystać?:
  sprawdź warunki licencji
• Rodzaj zawartości:
  obrazy