Szczegóły obiektu: Differential scanning fluorimetry followed by microscale thermophoresis and/or isothermal titration calorimetry as an efficient tool for ligand screening

Instytucja dostarczająca:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Opis

Title:
Differential scanning fluorimetry followed by microscale thermophoresis and/or isothermal titration calorimetry as an efficient tool for ligand screening
Creator:
Winiewska-Szajewska, Maria;
Poznański, Jarosław
Description:
Various biophysical and biochemical techniques have been developed to measure the affinity of interacting molecules. This review analyzes the combination of three methods: differential scanning fluorimetry as the initial high-throughput screening technique and microscale thermophoresis and isothermal titration calorimetry as complementary methods to quantify binding affinity. The presented work is the first to detailed compare the strengths and flaws of these three specific methods, as well as their application possibilities and complementarity. The fundamentals of these methods will be covered, including the most often-used models for characterizing observable phenomena and an emphasis on methods for analyzing data. A comprehensive review of numerous approaches to data analysis found in the literature is additionally provided, with the benefits and drawbacks of each, as well as the pitfalls and related concerns. Finally, examples of different systems will be presented, and methods used and some discrepancies in results will be described and discussed.
Object availability:
Dostęp otwarty
Date:
2025.02.13
Type:
Article;
PeerReviewed
Language:
angielski
Format:
application/pdf
Relation: http://eprints.ibb.waw.pl/2533 ;https://link.springer.com/article/10.1007/s12551-025-01280-3 ;https://doi.org/10.1007/s12551-025-01280-3
Subject:
Q Science (General)
Identifier: http://eprints.ibb.waw.pl/2533/1/s12551-025-01280-3.pdf ;
Winiewska-Szajewska, Maria and Poznański, Jarosław (2025) Differential scanning fluorimetry followed by microscale thermophoresis and/or isothermal titration calorimetry as an efficient tool for ligand screening. Biophysical Reviews, 17 . pp. 199-223.
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Szczegóły obiektu: Differential scanning fluorimetry followed by microscale thermophoresis and/or isothermal titration calorimetry as an efficient tool for ligand screening

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