Opis

• Tytuł:
  Time-Course Microarray Analysis Reveals Differences between Transcriptional Changes in Tomato Leaves Triggered by Mild and Severe Variants of Potato Spindle Tuber Viroid
• Twórca:
  Więsyk, Aneta;
  Iwanicka-Nowicka, Roksana;
  Fogtman, Anna;
  Zagórski-Ostoja, Włodzimierz;
  Góra-Sochacka, Anna
• Opis:
  Viroids are small non-capsidated non-coding RNA replicons that utilize host factors for efficient propagation and spread through the entire plant. They can incite specific disease symptoms in susceptible plants. To better understand viroid-plant interactions, we employed microarray analysis to observe the changes of gene expression in ‘Rutgers’ tomato leaves in response to the mild (M) and severe (S23) variants of potato spindle tuber viroid (PSTVd). The changes were analyzed over a time course of viroid infection development: (i) the pre-symptomatic stage, (ii) early symptoms, (iii) full spectrum of symptoms and (iv) the so-called ‘recovery’ stage, when stem regrowth was observed in severely affected plants. Gene expression profiles differed depending on stage of infection and variant. In S23-infected plants, the expression of over 3,000 genes was affected, while M-infected plants showed 3-fold fewer differentially expressed genes, only 20% of which were specific to the M variant. The differentially expressed genes included many genes related to stress; defense; hormone metabolism and signaling; photosynthesis and chloroplasts; cell wall; RNA regulation, processing and binding; protein metabolism and modification and others. The expression levels of several genes were confirmed by nCounter analysis.
• Dostępność obiektu:
  Dostęp otwarty
• Data:
  2018.05.17
• Typ:
  Article;
  PeerReviewed
• Język:
  angielski
• Format:
  application/pdf
• Relacja: http://eprints.ibb.waw.pl/1530 ;http://doi.org/10.3390/v10050257 ;doi:10.3390/v10050257
• Temat i słowa kluczowe:
  QR355 Virology
• Identyfikator: http://eprints.ibb.waw.pl/1530/1/viruses-10-00257-1.pdf ;
  Więsyk, Aneta and Iwanicka-Nowicka, Roksana and Fogtman, Anna and Zagórski-Ostoja, Włodzimierz and Góra-Sochacka, Anna (2018) Time-Course Microarray Analysis Reveals Differences between Transcriptional Changes in Tomato Leaves Triggered by Mild and Severe Variants of Potato Spindle Tuber Viroid. Viruses, 10 (5). p. 257. ISSN 1999-4915
• Dostawca danych:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Czy mogę z tego obiektu skorzystać?:
  poproś o pozwolenie
• Rodzaj zawartości:
  pozostałe