Opis

• Tytuł:
  Transcriptional cytokine responses associated with pathological outcomes in chickens experimentally infected with pigeon variant of avian avulavirus type 1
• Twórca:
  Olszewska-Tomczyk, Monika;
  Jasik, Agnieszka
• Opis:
  Background: Newcastle disease (ND) caused by virulent avian avulavirus 1 (AAvV-1) within the family paramyxoviridae, affects variety of avian species around the globe as well as responsible for significant economic losses in the poultry industry.Methods: Transcriptional response of selected cytokines associated with pathological outcomes were studied on chickens after experimental infection with three virulent isolates of pigeon variant avian avulavirus type 1 (PAvV-1). The specific pathogen free (SPF) chickens were used in the study, after virus inoculation via the intranasal route virus, observed daily for clinical symptoms. On day 3 of post infection, the experiment was terminated and then anatomopathogical and histopathological changes, virus load and transcriptional cytokine response were determined in the spleen.Results: For the study, four cytokines were chosen: interferons (IFN-β, IFN-γ) and interleukins (IL-2, IL-6). The increased transcriptional levels of cytokine expression were associated with viral gene expression and marked lesions. The increase in the mRNA levels of IL-6 was most significant in chickens with clinical symptoms. The combined up-regulations of IFN-γ and IL-6 mRNA expression were detected simultaneously with infiltration of macrophages in the histopathology analysis. The RNA expression of IL-2 and slight up-regulation of IFN-β were only in chickens infected with isolate closely related to poultry isolates in the phylogenetic study.Conclusions: The difference in the mRNA expression levels of cytokines may help to elucidate mechanisms of immune responses in birds infected with different virulent strains of avian avulavirus type 1.
• Dostępność obiektu:
  Dostęp otwarty
• Prawa:
  Prawa autorskie (c) 2021 Translational Research In Veterinary Science
• Data:
  2021.01.23
• Typ:
  info:eu-repo/semantics/article;
  info:eu-repo/semantics/publishedVersion
• Język:
  angielski
• Źródło:
  Translational Research in Veterinary Science; Vol. 3 No. 1 (2020); 9-28;
  Translational Research In Veterinary Science; Tom 3 Nr 1 (2020); 9-28;
  2657-4489
• Format:
  application/pdf
• Wydawca:
  Nicolaus Copernicus University in Toruń
• Relacja: https://apcz.umk.pl/TRVS/article/view/TRVS.2020.001/27912
• Temat i słowa kluczowe:
  chicken;
  pigeon variant of avian avulavirus type 1;
  innate immune response;
  cytokine;
  pathogenicity;
  viral loads
• Identyfikator: https://apcz.umk.pl/TRVS/article/view/TRVS.2020.001
• Dostawca danych:
  Akademicka Platforma Czasopism
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