Opis

• Tytuł:
  Biochemical characteristics of myofibrillar proteins of the fish parasite Isoparorchis hypselobagri (Trematoda) as survival traits in an oxygen-rich environment
• Twórca:
  Abbasi, Usma;
  Ahmad, Riaz;
  Hasnain, Absar-ul
• Opis:
  We have investigated biochemical properties of myofibrillar proteins of the digenetic trematode Isoparorchis hypselobagri, which correlate with its survival in the oxygen-rich swim bladder of its host catfish (Wallago attu). The polypeptide composition of the trematode’s natural actomyosin (NAM) was striated-muscle-like, with the exception that a 98-kD polypeptide corresponding to paramyosin also existed in its sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) profiles. The profiles of immunoprecipitated NAM of the trematode support these inferences. Ca2+-sensitivity of myofibrillar contractility and Mg2+-ATPase activity of I. hypselobagri resembled troponin-linked calcium regulation of the host striated muscle. Myofibrillar permeability to water influx was insensitive to calcium chelation at neutral pH. However, the host swim bladder myofibrils displayed smooth-muscle-like polypeptide composition, pH dependence of contractility, Ca2+-sensitivity, ATPase activities, and inactivation kinetics. We propose 2 survival strategies that I. hypselobagri appears to have co-evolved: (i) fast-muscle-like musculature with exceptionally high contractility or ATPase activity; and (ii) type-II myosin resembling the host muscle in functional plasticity.
• Dostępność obiektu:
  Dostęp otwarty
• Data:
  2013.02.14
• Typ:
  info:eu-repo/semantics/article;
  info:eu-repo/semantics/publishedVersion;
  Recenzowany artykuł
• Język:
  angielski
• Źródło:
  Biological Letters; Vol. 49 No. 1 (2012); 45-58;
  Biological Letters; Tom 49 Nr 1 (2012); 45-58;
  1734-7467;
  1644-7700
• Format:
  application/pdf
• Wydawca:
  Adam Mickiewicz University Poznan
• Relacja: http://pressto.amu.edu.pl/index.php/BIOLET/article/view/v10120-012-0009-0/28657
• Temat i słowa kluczowe:
  Isoparorchis hypselobagri;
  immunoprecipitation;
  natural actomyosin;
  contractility;
  myofibrillar ATPase;
  oxygen-rich environment;
  Wallago attu
• Identyfikator: http://pressto.amu.edu.pl/index.php/BIOLET/article/view/v10120-012-0009-0
• Dostawca danych:
  Platforma Otwartych Czasopism Naukowych UAM
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