Description

• Title:
  Rapid method for Mycobacterium tuberculosis identification using electrospray ionization tandem mass spectrometry analysis of mycolic acids
• Creator:
  Szewczyk, Rafał;
  Kowalski, Konrad;
  Janiszewska-Drobińska, Beata;
  Druszczynska, Magdalena
• Contributor:
  Rafał Szewczyk - Faculty of Biology and Environmental Protection, University of Łodz;
  Konrad Kowalski - Institute of Immunology and Experimental Therapy, PAS;
  Beata Janiszewska-Drobińska - Specialized Hospital of Tuberculosis, Lung Diseases and Rehabilitation;
  Magdalena Druszczyńska - Faculty of Biology and Environmental Protection, University of Łódz
• Description:
  Mycolic acids (MAs), which play a crucial role in the architecture of mycobacterial cell walls, were analyzed using electrospray ionization tandem mass spectrometry. A targeted analysis based on the 10 most abundant and characteristic multiple reaction monitoring pairs was used to profile the crude fatty acid mixtures from Mtb and several nontuberculous mycobacterial strains. Comparative analysis yielded unique profiles for MAs, enabling the reliable identification of mycobacterial species. In a case-control study of tuberculosis (TB) and non-TB Polish patients, we demonstrated the potential diagnostic utility of our approach for the rapid diagnosis of active TB with sensitivity and specificity surpassing those of existing methods. This robust method allows the identification of TB-positive patients after 2 h of sample preparation in the case of direct sputum analysis or 10 days of culturing, both of which are followed by 1 min of liquid chromatography– tandem mass spectrometry analysis.
• Object availability:
  free access
• Rights:
  Uznanie autorstwa-Użycie niekomercyjne-Bez utworów zależnych 3.0 Polska;
  http://creativecommons.org/licenses/by-nc-nd/3.0/pl
• Date:
  2015.06.15;
  2013.05.11
• Type:
  Article
• Language:
  English
• Format:
  application/pdf
• Publisher:
  Elsevier Health Sciences
• Relation:
  Diagnostic Microbiology and Infectious Disease;Volume 76, Issue 3, July 2013
• Subject:
  Mycobacterium tuberculosis;
  Mycolic acids;
  Mass spectrometry;
  Liquid chromatography;
  lc-ms/ms;
  Multiple reaction monitoring;
  clinical diagnostics
• Identifier:
  1879-0070;
  http://hdl.handle.net/11089/9734 ;
  10.1016/j.diagmicrobio.2013.03.025
• Data provider:
  Repozytorium Uniwersytetu Łódzkiego
• Can I use it?:
  check license
• Type:
  image