Object's details: Development and study of perspective clinical properties of biologically active matrix composed of decellularized lyophilized human amniotic membrane in combination with platelet-derived growth factors and hyaluronic acid

Provider:Akademicka Platforma Czasopism

Description

Title:
Development and study of perspective clinical properties of biologically active matrix composed of decellularized lyophilized human amniotic membrane in combination with platelet-derived growth factors and hyaluronic acid
Creator:
Vyshnevska, H. O.;
Kakabadze, Z. Sh.;
Schnayder, S. A.;
Tiron, O. I.;
Dzygal, O. F.
Description:
Nowadays the development of novel biomaterials which would promote tissue lesions regeneration is one of the crucial tasks of regenerative medicine. In the current research we developed a biological active membrane composed of decellularized, lyophilized human amniotic membrane in combination with growth factors of platelet-rich plasma (PRP) and hyaluronic acid. On the basis of histological examination, scanning electron microscopic examination and DNA content analyses it was drawn a conclusion that Sodium Dodecyl Sulfate (SDS) (Sigma) solution and 1% Triton X-100 solution completely remove all existing cells of placenta and chorionic-amniotic membrane without impairment of their structure. After the processes of decellularization and lyophilization the DNA content was less than 2%. The PRP growth factors as the components biological matrix provide faster regeneration. The latter are fixed on the surface of cellular structure of decellularized, lyophilized human amniotic membrane by the reservoir of gelatinous hyaluronic acid
Object availability:
free access
Rights:
Prawa autorskie (c) 2019 © The Author (s)
Date:
2019.11.29
Type:
info:eu-repo/semantics/article;
info:eu-repo/semantics/publishedVersion
Language:
English
Source:
Journal of Education, Health and Sport; Vol. 9 No. 11 (2019); 410-418;
Journal of Education, Health and Sport; Tom 9 Nr 11 (2019); 410-418;
2391-8306
Format:
application/pdf
Publisher:
Nicolaus Copernicus University, Toruń, Poland
Relation: https://apcz.umk.pl/JEHS/article/view/JEHS.2019.09.11.038/26538
Subject:
amniotic membrane;
growth factors;
hyaluronic acid;
morphology;
scanning electron microscopy
Identifier: https://apcz.umk.pl/JEHS/article/view/JEHS.2019.09.11.038
Data provider:
Akademicka Platforma Czasopism

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