Description

• Title:
  A Simplified Method for Simultaneous Determination of α-Lipoic Acid and Low-Molecular-Mass Thiols in Human Plasma
• Creator:
  Borowczyk, Kamila;
  Olejarz, Patrycja;
  Chwatko, Grażyna;
  Szylberg, Marcin;
  Głowacki, Rafał
• Contributor:
  Department of Environmental Chemistry, Faculty of Chemistry, University of Lodz, Pomorska 163, 90-236 Łódź, Poland;
  Rehabilitation Center “Kraszewski”, Kraszewskiego 7/9, 93-161 Łódź, Poland
• Description:
  α-Lipoic acid, glutathione, cysteine, and cysteinylglycine can be applied as therapeutic agents in civilization diseases such as diabetes mellitus, cardiovascular diseases, and cancers. On the other hand, a higher concentration of homocysteine can result in health problems and has been indicated as an independent risk factor for cardiovascular disease and accelerated atherosclerosis. Here, the first simplified HPLC-UV assay that enables simultaneous determination of α-lipoic acid and low-molecular-mass thiols in plasma, reduces the number of steps, shortens the total time of sample preparation, and limits the amount of single-use polypropylene laboratory materials is described. The assay is based on reversed-phase high performance liquid chromatography with UV detection and simultaneous reduction of disulfide bound with tris(2-carboxyethyl)phosphine and the selective pre-column derivatization of the thiol group with 1-benzyl-2-chloropyridinium bromide. Linearity in the detector responses for plasma samples were observed in ranges: 0.12–5.0 nmol mL−1 for α-lipoic acid; 2.0–20.0 nmol mL−1 for glutathione, cysteinylglycine, and homocysteine; and 40.0–400.0 for cysteine. The LODs for α-lipoic acid and low-molecular-mass thiols were 0.08 and 0.12 nmol mL−1 , respectively, while LOQs were 0.12 and 0.16 nmol mL−1 , respectively. The usefulness of the proposed method has been proven by its application to real samples.
• Object availability:
  free access
• Rights:
  Uznanie autorstwa 4.0 Międzynarodowe;
  http://creativecommons.org/licenses/by/4.0
• Date:
  2021.09.09;
  2020
• Type:
  Article
• Language:
  English
• Format:
  application/pdf
• Publisher:
  MDPI
• Relation:
  International Journal of Molecular Sciences;21
• Subject:
  α-lipoic acid;
  low-molecular-mass thiols;
  simultaneous reduction and derivatization;
  high performance liquid chromatography;
  ultraviolet detection
• Identifier:
  Borowczyk K, Olejarz P, Chwatko G, Szylberg M, Głowacki R. A Simplified Method for Simultaneous Determination of α-Lipoic Acid and Low-Molecular-Mass Thiols in Human Plasma. International Journal of Molecular Sciences. 2020; 21(3):1049. https://doi.org/10.3390/ijms21031049;
  1422-0067;
  http://hdl.handle.net/11089/39006 ;https://doi.org/10.3390/ijms21031049
• Data provider:
  Repozytorium Uniwersytetu Łódzkiego
• Can I use it?:
  check license
• Type:
  image