Description

• Title:
  The use of high-performance liquid chromatography with diode array detector for the determination of sulfide ions in human urine samples using pyrylium salts
• Creator:
  Zakrzewski, Robert;
  Zgagacz, W.;
  Urbaniak, Katarzyna;
  Chwatko, Grażyna;
  Nowicki, A.
• Contributor:
  Department of Environmental Chemistry, Faculty of Chemistry, University of Lodz, Poland;
  Department of Organic and Applied Chemistry, Faculty of Chemistry, University of Lodz, Poland
• Description:
  Hydrogen sulfide is a toxic gas involved in the regulation of some essential biological processes. A novel, precise, accurate and rapid method based on high-performance liquid chromatography with diode array detection for the determination of sulfide ions in human urine sample is proposed. The method involves the derivatization of sulfide with pyrylium salts – (2,4,6-triphenylpyrylium hydrogensulfate(VI) (L1) and 4-[p-(N,N-dimethylamino)phenyl]-2,6-diphenylpyrylium chlorate(VII) (LN1). The separation occurs on InfinityLab Poroshell 120 EC C18 column using acetonitrile and phosphate buffer as a mobile phase. The detectors utilized a wavelength of 371 or 580 nm. The calibration curves were linear in the range of 2–150 μmol L−1 and 1–50 μmol L−1 for L1 and LN1 derivatives, respectively. The samples were found to be stable from sample collection to final analysis. The method was successfully applied to samples from apparently healthy volunteers.
• Object availability:
  free access
• Rights:
  Uznanie autorstwa 4.0 Międzynarodowe;
  http://creativecommons.org/licenses/by/4.0
• Date:
  2021.08.25;
  2020
• Type:
  Article
• Language:
  English
• Format:
  application/pdf
• Publisher:
  Elsevier
• Relation:
  Journal of Chromatography B;1157
• Subject:
  jon siarczkowy;
  sole piryliowe;
  wysokosprawna chromatografia cieczowa (HPLC);
  detektor diodowy (DAD);
  mocz;
  Sulfide ions;
  Pyrylium salts;
  High performance liquid chromatography with diode detector (DAD);
  Human urine sample
• Identifier:
  1570-0232;
  http://hdl.handle.net/11089/38840 ;
  1873-376X;
  10.1016/j.jchromb.2020.122309
• Data provider:
  Repozytorium Uniwersytetu Łódzkiego
• Can I use it?:
  check license
• Type:
  image