Object's details: Co-Fermentation and Genomic Insights into Lactic Acid Bacteria for Enhanced Propionic Acid Production Using a Non-GMO Approach

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Co-Fermentation and Genomic Insights into Lactic Acid Bacteria for Enhanced Propionic Acid Production Using a Non-GMO Approach
Creator:
Stasiak-Różańska, Lidia;
Gawor, Jan;
Piwowarek, Kamil;
Fabiszewska, Agata;
Aleksandrzak-Piekarczyk, Tamara
Description:
Propionic acid (PA) is an important organic acid with applications in food preservation, feed additives, and bio-based chemical production. While industrial PA is mostly derived from petrochemical processes, sustainable microbial alternatives are gaining attention. In this study, we explored a co-fermentation strategy using lactic acid bacteria (LAB) with complementary metabolic capabilities to enhance PA biosynthesis via the 1,2-propanediol (PDO) pathway. Genome-based screening identified a metabolic division between strains capable of producing PDO (e.g., Carnobacterium maltaromaticum IBB3447) and those converting PDO to PA (e.g., Levilactobacillus brevis IBB3735). Notably, we discovered that C. maltaromaticum IBB3447 is capable of PDO 24 biosynthesis, a function previously undescribed in this species. Phenotypic assays confirmed glycerol metabolism and acid tolerance among strains. In co-culture fermentation trials, the highest PA concentration (6.87 mM) was achieved using simultaneous fermentation in a fructose–sorbitol–glucose (FRC-SOR-GLC) medium, accompanied by prior PDO accumulation (up to 13.13 mM). No single strain produced PA independently, confirming that metabolic cooperation is required. These findings reveal a novel LAB-based bioprocess for sustainable PA and PDO production, using cross-feeding interactions and the valorization of industrial waste streams. The study supports future optimization and scale-up for circular bioeconomy applications.
Object availability:
free access
Date:
2025.04.29
Type:
Article;
PeerReviewed
Language:
English
Format:
application/pdf
Relation: http://eprints.ibb.waw.pl/2563 ;http://doi.org/10.3390/foods14091573 ;doi:10.3390/foods14091573
Subject:
QR Microbiology
Identifier: http://eprints.ibb.waw.pl/2563/1/foods-14-01573-v2.pdf ;
Stasiak-Różańska, Lidia and Gawor, Jan and Piwowarek, Kamil and Fabiszewska, Agata and Aleksandrzak-Piekarczyk, Tamara (2025) Co-Fermentation and Genomic Insights into Lactic Acid Bacteria for Enhanced Propionic Acid Production Using a Non-GMO Approach. Foods, 14 (9). p. 1573. ISSN 2304-8158
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Similar objects

Co-Fermentation and Genomic Insights into Lactic Acid Bacteria for Enhanced Propionic Acid Production Using a Non-GMO Approach

Creator:Stasiak-Różańska, Lidia | Gawor, Jan | Piwowarek, Kamil | Fabiszewska, Agata | Aleksandrzak-Piekarczyk, Tamara

Date:2025.04.29

Type:other

Lactose and β-Glucosides Metabolism and Its Regulation in Lactococcus lactis: A Review.

Creator:Aleksandrzak-Piekarczyk, Tamara

Date:2013.01

Type:text

The Presence of Plasmids in Lactococcus lactis IL594 Determines Changes in the Host Phenotype and Expression of Chromosomal Genes

Creator:Kosiorek, Katarzyna | Koryszewska-Bagińska, Anna | Skoneczny, Marek | Stasiak-Różanska, Lidia | Aleksandrzak-Piekarczyk, Tamara

Date:2023.01.02

Type:other

Genome sequences of polar Carnobacterium maltaromaticum strains 2857 and 2862 with genes for glycerol and 1,2-propanediol pathways

Creator:Aleksandrzak-Piekarczyk, Tamara | Grzesiak, Jakub | Gawor, Jan | Kosiorek, Katarzyna

Date:2025.05.27

Type:other

Genomic Analysis of Cronobacter condimenti s37: Identification of Resistance and Virulence Genes and Comparison with Other Cronobacter and Closely Related Species

Date:2024.08.07

Type:other

Polar-Region Soils as Novel Reservoir of Lactic Acid Bacteria from the Genus Carnobacterium

Creator:Kosiorek, Katarzyna | Grzesiak, Jakub | Gawor, Jan | Sałańska, Agnieszka | Aleksandrzak-Piekarczyk, Tamara

Date:2024

Type:other

The Lactococcal dgkB (yecE) and dxsA Genes for Lipid Metabolism Are Involved in the Resistance to Cell Envelope-Acting Antimicrobials

Creator:Tymoszewska, Aleksandra | Aleksandrzak-Piekarczyk, Tamara

Date:2021

Type:other

Badania nad mechanizmami działania i rozwoju oporności na bakteriocyny klasy II u bakterii Gram-dodatnich

Creator:Tymoszewska, Aleksandra | Aleksandrzak-Piekarczyk, Tamara

Date:2024.07.01

Type:other

Federacja Bibliotek Cyfrowych

Object's details: Co-Fermentation and Genomic Insights into Lactic Acid Bacteria for Enhanced Propionic Acid Production Using a Non-GMO Approach

Description

Similar objects