Object's details: Increased activity of the sterol branch of the mevalonate pathway elevates glycosylation of secretory proteins and improves antifungal properties of Trichoderma atroviride.

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Increased activity of the sterol branch of the mevalonate pathway elevates glycosylation of secretory proteins and improves antifungal properties of Trichoderma atroviride.
Creator:
Graczyk, Sebastian;
Perlinska-Lenart, Urszula;
Górka-Nieć, Wioletta;
Lichota, Renata;
Piłsyk, Sebastian;
Zembek, Patrycja;
Lenart, Jacek;
Bernat, Przemysław;
Gryz, Elzbieta;
Augustyniak, Justyna;
Palamarczyk, Grażyna;
Kruszewska, Joanna S.
Description:
Some Trichoderma spp. have an ability to inhibit proliferation of fungal plant pathogens in the soil. Numerous compounds with a proven antifungal activity are synthesized via the terpene pathway. Here, we stimulated the activity of the mevalonate pathway in T. atroviride P1 by expressing the Saccharomyces cerevisiae ERG20 gene coding for farnesyl pyrophosphate (FPP) synthase, a key enzyme of this pathway. ERG20-expressing Trichoderma strains showed higher activities of FPP synthase and squalene synthase, the principal recipient of FPP in the mevalonate pathway. We also observed activation of dolichyl phosphate mannose (DPM) synthase, an enzyme in protein glycosylation, and significantly increased O- and N-glycosylation of secreted proteins. The hyper-glycosylation of secretory hydrolases could explain their increased activity observed in the ERG20 transformants. Analysis of the antifungal properties of the new strains revealed that the hydrolases secreted by the transformants inhibited growth of a plant pathogen, Pythium ultimum more efficiently compared to the control strain. Consequently, the biocontrol activity of the transgenic strains, determined as their ability to protect bean seeds and seedlings against harmful action of P. ultimum, was also improved substantially.
Object availability:
free access
Date:
2020
Type:
Article;
PeerReviewed
Language:
English
Format:
application/pdf
Publisher:
Elsevier
Relation: http://eprints.ibb.waw.pl/1817 ;https://www.sciencedirect.com/science/article/pii/S1087184519302221?via%3Dihub ;
10.1016/j.fgb.2020.103334
Subject:
QH301 Biology
Identifier: http://eprints.ibb.waw.pl/1817/1/FGB-2020.pdf ;
Graczyk, Sebastian and Perlinska-Lenart, Urszula and Górka-Nieć, Wioletta and Lichota, Renata and Piłsyk, Sebastian and Zembek, Patrycja and Lenart, Jacek and Bernat, Przemysław and Gryz, Elzbieta and Augustyniak, Justyna and Palamarczyk, Grażyna and Kruszewska, Joanna S. (2020) Increased activity of the sterol branch of the mevalonate pathway elevates glycosylation of secretory proteins and improves antifungal properties of Trichoderma atroviride. Fungal Genetics and Biology, 137 . p. 103334. ISSN 1087-1845
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: Increased activity of the sterol branch of the mevalonate pathway elevates glycosylation of secretory proteins and improves antifungal properties of Trichoderma atroviride.

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