Description

• Title:
  Systematic bioinformatics and experimental validation of yeast complexes reduces the rate of attrition during structural investigations.
• Creator:
  Brooks, MA;
  Gewartowski, Kamil;
  Mitsiki, E;
  Létoquart, J;
  Pache, RA;
  Billier, Y;
  Bertero, M;
  Corréa, M;
  Czarnocki-Cieciura, Mariusz;
  Dadlez, Michal;
  Henriot, V;
  Lazar, N;
  Delbos, L;
  Lebert, D;
  Piwowarski, Jan;
  Rochaix, P;
  Böttcher, B;
  Serrano, L;
  Séraphin, Bertrand;
  van Tilbeurgh, H;
  Aloy, P;
  Perrakis, A;
  Dziembowski, Andrzej
• Description:
  For high-throughput structural studies of protein complexes of composition inferred from proteomics data, it is crucial that candidate complexes are selected accurately. Herein, we exemplify a procedure that combines a bioinformatics tool for complex selection with in vivo validation, to deliver structural results in a medium-throughout manner. We have selected a set of 20 yeast complexes, which were predicted to be feasible by either an automated bioinformatics algorithm, by manual inspection of primary data, or by literature searches. These complexes were validated with two straightforward and efficient biochemical assays, and heterologous expression technologies of complex components were then used to produce the complexes to assess their feasibility experimentally. Approximately one-half of the selected complexes were useful for structural studies, and we detail one particular success story. Our results underscore the importance of accurate target selection and validation in avoiding transient, unstable, or simply nonexistent complexes from the outset.
• Object availability:
  free access
• Date:
  2010
• Type:
  Article;
  PeerReviewed
• Language:
  English
• Format:
  other;
  application/pdf
• Publisher:
  Cell press
• Relation: http://eprints.ibb.waw.pl/134 ;http://vls2.icm.edu.pl/cgi-bin/sciserv.pl?collection=elsevier&journal=09692126&issue=v18i0009
• Subject:
  QH301 Biology
• Identifier: http://eprints.ibb.waw.pl/134/4/20826334 ;http://eprints.ibb.waw.pl/134/5/structure.pdf ;
  Brooks, MA and Gewartowski, Kamil and Mitsiki, E and Létoquart, J and Pache, RA and Billier, Y and Bertero, M and Corréa, M and Czarnocki-Cieciura, Mariusz and Dadlez, Michal and Henriot, V and Lazar, N and Delbos, L and Lebert, D and Piwowarski, Jan and Rochaix, P and Böttcher, B and Serrano, L and Séraphin, Bertrand and van Tilbeurgh, H and Aloy, P and Perrakis, A and Dziembowski, Andrzej (2010) Systematic bioinformatics and experimental validation of yeast complexes reduces the rate of attrition during structural investigations. STRUCTURE, 18 (9). pp. 1075-1082. ISSN X
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  seek permission
• Type:
  other