Object's details: B-cell chronic lymphocytic leukemia-associated nuclear antigens

Provider:Repozytorium Uniwersytetu Łódzkiego

Description

Title:
B-cell chronic lymphocytic leukemia-associated nuclear antigens
Creator:
Rogalinska, Malgorzata;
Błoński, Jerzy Z.;
Robak, Tadeusz;
Kiliańska, Zofia M.
Contributor:
Małgorzata Rogalińska - University of Łódź, Department of Cytobiochemistry;
Jerzy Z. Błoński - Medical University of Łódź, Department of Hematology;
Tadeusz Robak - Medical University of Łódź, Department of Hematology;
Zofia M. Kiliańska - University of Łódź, Department of Cytobiochemistry
Description:
One- and two-dimensional polyacrylamide gel electrophoresis were used to compare the composition of nuclear polypeptides from normal and В-cell chronic lymphocytic leukemia mononuclear cells. Against two electrophoretically-specific nuclear proteins with molecular weight of 38/39 and 44/46 kD a from leukemic cells rabbit sera were obtained. As it was analyzed by Western blot technique the available antisera recognized the 38/39 kDa antigen in 53 of the 56 (94.6%), while the 44/46 kDa in 46 of the 49 (93.9%) of examined В-CLL nuclear fraction preparations, but not in normal ones. The pi values of described leukaemia-specific antigens were determined; p38/39 had pi in the range of pH 6.55 -7.00 and p44/46 - in the range of pH 6.2-6.4.;
Zadanie pt. „Digitalizacja i udostępnienie w Cyfrowym Repozytorium Uniwersytetu Łódzkiego kolekcji czasopism naukowych wydawanych przez Uniwersytet Łódzki” nr 885/P-DUN/2014 dofinansowane zostało ze środków MNiSW w ramach działalności upowszechniającej naukę.
Object availability:
free access
Date:
2015.10.09;
2008
Type:
Article
Language:
English
Format:
application/pdf
Publisher:
Wydawnictwo Uniwersytetu Łódzkiego
Relation:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica;4
Subject:
B-CLL specific antigens;
electrophoresis;
immunoblotting
Identifier:
1730-2366;
http://hdl.handle.net/11089/12336
Data provider:
Repozytorium Uniwersytetu Łódzkiego

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Description

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