Description

• Title:
  The differences in thermal profiles between normal and leukemic cells exposed to anticancer drug evaluated by differential scanning calorimetry
• Creator:
  Góralski, Paweł;
  Rogalinska, Malgorzata;
  Błoński, Jerzy Z.;
  Pytel, Edyta;
  Robak, Tadeusz;
  Kiliańska, Zofia M.;
  Piekarski, Henryk
• Contributor:
  University of Lodz, Department of Physical Chemistry;
  University of Lodz, Department of Cytobiochemistry;
  Medical University of Lodz, Department of Hematology;
  kchfpiek@uni.lodz.pl
• Description:
  Chronic lymphocytic leukemia (CLL) is a heterogenous disease with an imbalance between apoptosis and cell proliferation. Therefore, the main goal in CLL therapy is to induce apoptosis and effectively support this process in transformed B lymphocytes. In the current study, we have compared differential scanning calorimetry (DSC) profiles of nuclei isolated from CLL cells and normal mononuclear cells exposed to cladribine or fludarabine combined with mafosfamide (CM; FM), and additionally to CM combined with monoclonal antibody—rituximab (RCM) for 48 h, as well as in culture medium only (controls). Under current study, the mononuclear cells from peripheral blood (PBMCs) of healthy individuals have been included. The obtained results have shown the presence of thermal transition at 95 ± 5 °C in most of nuclear preparations (92.2 %) isolated from blood of CLL patients. This thermal characteristic parameter was changed after drug exposure, however, to a different extent. These thermal changes were accompanied by the decrease of cell viability, an elevation of apoptosis rate and the changes in expression/proteolysis of poly(ADP-ribose)polymerase-1—main marker of apoptosis. Importantly, in DSC profiles of nuclear preparations of PBMCs from blood of healthy donors exposed to investigated drug combinations and control CLL cells, the lack of such changes was observed. Our results confirmed that DSC technique complemented with other biological approaches could be helpful in tailoring therapy for CLL patients.;
  Research was sponsored by Grant from the Polish National Science Centre (No. 2011/01/B/NZ/0102); Results of presented study were partially presented in oral presentation on 2nd Central and Eastern European Conference on Thermal Analysis and Calorimetry in Vilnius, Lithuania, 2013
• Object availability:
  free access
• Rights:
  Uznanie autorstwa 3.0 Polska;
  http://creativecommons.org/licenses/by/3.0/pl
• Date:
  2016.03.23;
  2014
• Type:
  Article
• Language:
  English
• Format:
  application/pdf
• Publisher:
  Springer Netherlands
• Relation:
  Journal of Thermal Analysis and Calorimetry;2;
  118
• Subject:
  Chronic lymphocytic leukemia;
  Differential scanning calorimetry;
  Anti-leukemic tailored therapy;
  Purine analogs;
  Mafosfamide;
  Rituximab
• Identifier:
  1388-6150;
  http://hdl.handle.net/11089/17526 ;
  1588-2926;
  10.1007/s10973-014-3957-2
• Data provider:
  Repozytorium Uniwersytetu Łódzkiego
• Can I use it?:
  check license
• Type:
  image