Description

• Title:
  Visfatin impact on the proteome of porcine luteal cells during implantation
• Creator:
  Kopij, Grzegorz;
  Kiezun, Marta;
  Gudelska, Marlena;
  Dobrzyn, Kamil;
  Zarzecka, Barbara;
  Rytelewska, Edyta;
  Zaobidna, Ewa;
  Świderska, Bianka;
  Malinowska, Agata;
  Rak, Agnieszka;
  Kaminski, Tadeusz;
  Smolinska, Nina
• Description:
  Visfatin (VIS) is a hormone belonging to the adipokines’ group secreted mainly by the adipose tissue. VIS plays a crucial role in the control of energy homeostasis, inflammation, cell differentiation, and angiogenesis. VIS expression was confirmed in the hypothalamic–pituitary–gonadal (HPG) axis structures, as well as in the uterus, placenta, and conceptuses. We hypothesised that VIS may affect the abundance of proteins involved in the regulation of key processes occurring in the corpus luteum (CL) during the implantation process in pigs. In the present study, we performed the high-throughput proteomic analysis (liquid chromatography with tandem mass spectrometry, LC–MS/MS) to examine the in vitro influence of VIS (100 ng/mL) on differentially regulated proteins (DRPs) in the porcine luteal cells (LCs) on days 15–16 of pregnancy (implantation period). We have identified 511 DRPs, 276 of them were up-regulated, and 235 down-regulated in the presence of VIS. Revealed DRPs were assigned to 162 gene ontology terms. Western blot analysis of five chosen DRPs, ADAM metallopeptidase with thrombospondin type 1 motif 1 (ADAMTS1), lanosterol 14-α demethylase (CYP51A1), inhibin subunit beta A (INHBA), notch receptor 3 (NOTCH3), and prostaglandin E synthase 2 (mPGES2) confirmed the veracity and accuracy of LC–MS/MS method. We indicated that VIS modulates the expression of proteins connected with the regulation of lipogenesis and cholesterologenesis, and, in consequence, may be involved in the synthesis of steroid hormones, as well as prostaglandins’ metabolism. Moreover, we revealed that VIS affects the abundance of protein associated with ovarian cell proliferation, differentiation, and apoptosis, as well as CL new vessel formation and tissue remodelling. Our results suggest important roles for VIS in the regulation of ovarian functions during the peri-implantation period.
• Object availability:
  free access
• Date:
  2024.06.25
• Type:
  Article;
  PeerReviewed
• Language:
  English
• Format:
  application/pdf
• Relation: http://eprints.ibb.waw.pl/2414 ;http://doi.org/10.1038/s41598-024-65577-1 ;doi:10.1038/s41598-024-65577-1
• Subject:
  QL Zoology;
  SF Animal culture
• Identifier: http://eprints.ibb.waw.pl/2414/1/s41598-024-65577-1.pdf ;
  Kopij, Grzegorz and Kiezun, Marta and Gudelska, Marlena and Dobrzyn, Kamil and Zarzecka, Barbara and Rytelewska, Edyta and Zaobidna, Ewa and Świderska, Bianka and Malinowska, Agata and Rak, Agnieszka and Kaminski, Tadeusz and Smolinska, Nina (2024) Visfatin impact on the proteome of porcine luteal cells during implantation. Scientific Reports, 14 (1). p. 14625. ISSN 2045-2322
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  seek permission
• Type:
  other