Description

• Title:
  Proteomic analysis of carp seminal plasma provides insights into the immune response to bacterial infection of the male reproductive system
• Creator:
  Dietrich, Mariola A.;
  Adamek, Mikołaj;
  Teitge, Felix;
  Teich, Lukas;
  Jung-Schroers, Verena;
  Malinowska, Agata;
  Świderska, Bianka;
  Rakus, Krzysztof;
  Kodzik, Natalia;
  Chadzińska, Magdalena;
  Karol, Halina;
  Liszewska, Ewa;
  Ciereszko, Andrzej
• Description:
  Aeromonas salmonicida is recognized as a significant bacterial pathogen in ulcerative disease of cyprinid fish. However, the mechanism of immunity to these bacteria in common carp is still not well understood, especially the immune regulation in the gonad to bacterial infection. The aims of our study were to analyze changes in the seminal plasma proteome following A. salmonicida infection in carp males. The observed pathological changes in the tissue (liver, spleen, kidney and testis) morphology and upregulation of immune-related genes (tnfa2, il6a) confirmed the successful infection challenge. Using mass spectrometry-based label-free quantitative proteomics, we identified 1402 seminal plasma proteins, and 44 proteins (20 up- and 24 downregulated) were found to be differentially abundant between infected and control males. Most differentially abundant proteins were involved in the immune response mechanisms, such as acute phase response, complement activation and coagulation, inflammation, lipid metabolism, cell-cell and cell-matrix adhesion, creatine-phosphate biosynthesis and germ cell-Sertoli cell junction signaling. Bacterial infection also caused profound changes in expression of selected genes in the testis and hematopoietic organs, which contributed to changes in seminal proteins. The altered seminal proteins and bacterial proteins in seminal plasma may serve as valuable markers of infection in the testis.
• Object availability:
  free access
• Date:
  2022.07.09
• Type:
  Article;
  PeerReviewed
• Language:
  English
• Format:
  application/pdf
• Publisher:
  Elsevier
• Relation: http://eprints.ibb.waw.pl/2171 ;http://doi.org/10.1016/j.fsi.2022.07.019 ;doi:10.1016/j.fsi.2022.07.019
• Subject:
  QL Zoology
• Identifier: http://eprints.ibb.waw.pl/2171/1/1-s2.0-S1050464822004089-main.pdf ;
  Dietrich, Mariola A. and Adamek, Mikołaj and Teitge, Felix and Teich, Lukas and Jung-Schroers, Verena and Malinowska, Agata and Świderska, Bianka and Rakus, Krzysztof and Kodzik, Natalia and Chadzińska, Magdalena and Karol, Halina and Liszewska, Ewa and Ciereszko, Andrzej (2022) Proteomic analysis of carp seminal plasma provides insights into the immune response to bacterial infection of the male reproductive system. Fish & Shellfish Immunology, 127 . pp. 822-835. ISSN 10504648
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  seek permission
• Type:
  other