Description

• Title:
  Polystyrene nanoparticles: the mechanism of their genotoxicity in human peripheral blood mononuclear cells
• Creator:
  Malinowska, Kinga;
  Bukowska, Bożena;
  Piwoński, Ireneusz;
  Foksiński, Marek;
  Kisielewska, Aneta;
  Zarakowska, Ewelina;
  Gackowski, Daniel;
  Sicińska, Paulina
• Contributor:
  Department of Biophysics of Environmental Pollution, Faculty of Biology and Environmental Protection, University of Lodz;
  Department of Materials Technology and Chemistry, Faculty of Chemistry, University of Lodz;
  Department of Clinical Biochemistry, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń;
  kinga.malinowska@edu.uni.lodz.pl
• Description:
  Plastic nanoparticles are widely spread in the biosphere, but health risk associated with their effect on the human organism has not yet been assessed. The purpose of this study was to determine the genotoxic potential of non-functionalized polystyrene nanoparticles (PS-NPs) of different diameters of 29, 44, and 72nm in human peripheral blood mononuclear cells (PBMCs) (in vitro). To select non-cytotoxic concentrations of tested PS-NPs, we analyzed metabolic activity of PBMCs incubated with these particles in concentrations ranging from 0.001 to 1000 ug/mL. Then, PS-NPs were used in concentrations from 0.0001 to 100 ug/mL and incubated with tested cells for 24 h. Physico-chemical properties of PS-NPs in media and suspension were analyzed using dynamic light scattering (DLS), atomic force microscopy (AFM), scanning electron microscopy (SEM) and zeta potential. For the first time, we investigated the mechanism of genotoxic action of PS-NPs based on detection of single/double DNA strand-breaks and 8-oxo-2'-deoxyguanosine (8-oxodG) formation, as well as determination of oxidative modification of purines and pyrimidines and repair efficiency of DNA damage. Obtained results have shown that PS-NPs caused a decrease in PBMCs metabolic activity, increased single/double-strand break formation, oxidized purines and pyrimidines and increased 8oxodG levels. The resulting damage was completely repaired in the case of the largest PS-NPs. It was also found that extent of genotoxic changes in PBMCs depended on the size of tested particles and their f-potential value.;
  Narodowe Centrum Nauki (NCN), Preludium 20 nr 2021/41/N/NZ7/02049
• Object availability:
  free access
• Rights:
  Uznanie autorstwa 4.0 Międzynarodowe;
  http://creativecommons.org/licenses/by/4.0
• Date:
  2023.11.22;
  2022
• Type:
  Article
• Language:
  English
• Format:
  application/pdf
• Publisher:
  Taylor & Francis
• Relation:
  Nanotoxicology;6-8;
  16
• Subject:
  polystyrene nanoparticles;
  peripheral blood mononuclear cells;
  DNA strand-breaks;
  oxidation of purines;
  oxidation of pyrimidines;
  8-oxo-20-deoxyguanosine
• Identifier:
  1743-5404;
  http://hdl.handle.net/11089/48404 ;
  10.1080/17435390.2022.2149360
• Data provider:
  Repozytorium Uniwersytetu Łódzkiego
• Can I use it?:
  check license
• Type:
  image