Object's details: Thioloxidoreductase HP0231 of Helicobacter pylori impacts HopQ-dependent CagA translocation

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Thioloxidoreductase HP0231 of Helicobacter pylori impacts HopQ-dependent CagA translocation
Creator:
Grzeszczuk, Magdalena J.;
Bocian-Ostrzycka, Katarzyna;
Banas, Hanna M.;
Roszczenko-Jasinska, Paula;
Malinowska, Agata;
Stralova, Hann;
Haas, Reiner;
Meyer, Thomas F.;
Jagusztyn-Krynicka, Elżbieta Katarzyna
Description:
Thioloxidoreductase HP0231 of Helicobacter pylori plays essential roles in gastric colonization and related gastric pathology. Comparative proteomics and analysis of complexes between HP0231 and its protein substrates suggested that several Hop proteins are its targets. HP0231 is a dimeric oxidoreductase that functions in an oxidizing Dsb (disulfide bonds) pathway of H. pylori. H. pylori HopQ possesses six cysteine residues, which generate three consecutive disulfide bridges. Comparison of the redox state of HopQ in wild-type cells to that in hp0231-mutated cells clearly indicated that HopQ is a substrate of HP0231. HopQ binds CEACAM1, 3, 5 and 6 (carcinoembryonic antigen-related cell adhesion molecules). This interaction enables T4SS-mediated translocation of CagA into host cells and induces host signaling. Site directed mutagenesis of HopQ (changing cysteine residues into serine) and analysis of the functioning of HopQ variants showed that HP0231 influences the delivery of CagA into host cells, in part through its impact on HopQ redox state. Introduction of a C382S mutation into HopQ significantly affects its reaction with CEACAM receptors, which disturbs T4SS functioning and CagA delivery. An additional effect of HP0231 on other adhesins and their redox state, resulting in their functional impairment, cannot be excluded.
Object availability:
free access
Date:
2018.08.07
Type:
Article;
NonPeerReviewed
Language:
English
Format:
application/msword
Relation: http://eprints.ibb.waw.pl/1604 ;https://ac.els-cdn.com/S1438422117305271/1-s2.0-S1438422117305271-main.pdf?_tid=604331cc-76b3-4c2a-baec-8ec402e1a3de&acdnat=1540806273_c5cca55320961e45316ea87de8ef2ab6
Subject:
QR Microbiology
Identifier: http://eprints.ibb.waw.pl/1604/1/grzeszczuk_draft.doc ;
Grzeszczuk, Magdalena J. and Bocian-Ostrzycka, Katarzyna and Banas, Hanna M. and Roszczenko-Jasinska, Paula and Malinowska, Agata and Stralova, Hann and Haas, Reiner and Meyer, Thomas F. and Jagusztyn-Krynicka, Elżbieta Katarzyna (2018) Thioloxidoreductase HP0231 of Helicobacter pylori impacts HopQ-dependent CagA translocation. International Journal of Medical Microbiology .
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: Thioloxidoreductase HP0231 of Helicobacter pylori impacts HopQ-dependent CagA translocation

Description

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