Object's details: Genomic and transcriptomic analysis of Ligilactobacillus salivarius IBB3154—in search of new promoters for vaccine construction

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Genomic and transcriptomic analysis of Ligilactobacillus salivarius IBB3154—in search of new promoters for vaccine construction
Creator:
Kobierecka, Patrycja;
Wyszyńska, Agnieszka;
Aleksandrzak-Piekarczyk, Tamara;
Sałańska, Agnieszka;
Gawor, Jan;
Bardowski, Jacek;
Jagusztyn-Krynicka, Elżbieta Katarzyna;
Polen, Tino
Description:
Transcriptomic analysis of the genome sequenced Ligilactobacillus salivarius strain IBB3154 grown at two different temperatures (37°C vs 42°C) identified differentially expressed genes involved in metabolic pathways, osmoregulation, and surface protein expression. Two highly expressed genes, sasA1 and sasA2, which encode cell wall-anchored proteins belonging to the serine-rich repeat protein group, were found to be temperature-inducible. Moonlighting proteins with various functions, such as glyceraldehyde 3-phosphate dehydrogenase, fructose-bisphosphate aldolase, elongation factor Tu, and enolase, were highly expressed at both temperatures. The efficiency of promoters has been confirmed by the β-glucuronidase activity test; however, temperature dependence was not detected. We also found that the P sasA1 promoter retained its activity in the presence of bile salts. Knowledge of promoters that are highly active in L. salivarius cells can be used to produce strains that are carriers of immunogenic proteins.
Object availability:
free access
Date:
2023.11.20
Type:
Article;
PeerReviewed
Language:
English
Format:
application/pdf
Relation: http://eprints.ibb.waw.pl/2346 ;http://doi.org/10.1128/spectrum.02844-23 ;doi:10.1128/spectrum.02844-23
Subject:
QR Microbiology
Identifier: http://eprints.ibb.waw.pl/2346/1/spectrum.02844-23 ;
Kobierecka, Patrycja and Wyszyńska, Agnieszka and Aleksandrzak-Piekarczyk, Tamara and Sałańska, Agnieszka and Gawor, Jan and Bardowski, Jacek and Jagusztyn-Krynicka, Elżbieta Katarzyna and Polen, Tino (2023) Genomic and transcriptomic analysis of Ligilactobacillus salivarius IBB3154—in search of new promoters for vaccine construction. Microbiology Spectrum, 11 (6). ISSN 2165-0497
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: Genomic and transcriptomic analysis of Ligilactobacillus salivarius IBB3154—in search of new promoters for vaccine construction

Description

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