Description

• Title:
  The angiotensin metabolite His-Leu is a strong copper chelator forming highly redox active species
• Creator:
  Wezynfeld, Nina Ewa;
  Sudzik, Dobromiła;
  Tobolska, Aleksandra;
  Makarova, Katerina;
  Stefaniak, Ewelina;
  Frączyk, Tomasz;
  Wawrzyniak, Urszula E.;
  Bal, Wojciech
• Description:
  His-Leu is a hydrolytic byproduct of angiotensin metabolism, whose concentration in the bloodstream could be at least micromolar. This encouraged us to investigate its Cu(II) binding properties and the concomitant redox reactivity. The Cu(II) binding constants were derived from isothermal titration calorimetry and potentiometry, while identities and structures of complexes were obtained from ultraviolet–visible, circular dichroism, and room-temperature electronic paramagnetic resonance spectroscopies. Four types of Cu(II)/His-Leu complexes were detected. The histamine-like complexes prevail at low pH. At neutral and mildly alkaline pH and low Cu(II):His-Leu ratios, they are superseded by diglycine-like complexes involving the deprotonated peptide nitrogen. At His-Leu:Cu(II) ratios of ≥2, bis-complexes are formed instead. Above pH 10.5, a diglycine-like complex containing the equatorially coordinated hydroxyl group predominates at all ratios tested. Cu(II)/His-Leu complexes are also strongly redox active, as demonstrated by voltammetric studies and the ascorbate oxidation assay. Finally, numeric competition simulations with human serum albumin, glycyl-histydyl-lysine, and histidine revealed that His-Leu might be a part of the low-molecular weight Cu(II) pool in blood if its abundance is >10 μM. These results yield further questions, such as the biological relevance of ternary complexes containing His-Leu.
• Object availability:
  free access
• Date:
  2024.06.15
• Type:
  Article;
  PeerReviewed
• Language:
  English
• Format:
  application/pdf
• Publisher:
  ACS Publications
• Relation: http://eprints.ibb.waw.pl/2408 ;https://pubs.acs.org/doi/10.1021/acs.inorgchem.4c01640 ;
  10.1021/acs.inorgchem.4c01640
• Subject:
  QD Chemistry;
  QH301 Biology
• Identifier: http://eprints.ibb.waw.pl/2408/1/The-angiotensin-metabolite-His-Leu-is-a-strong-copper-chelator-forming-highly-redox-active-species_2024.pdf ;
  Wezynfeld, Nina Ewa and Sudzik, Dobromiła and Tobolska, Aleksandra and Makarova, Katerina and Stefaniak, Ewelina and Frączyk, Tomasz and Wawrzyniak, Urszula E. and Bal, Wojciech (2024) The angiotensin metabolite His-Leu is a strong copper chelator forming highly redox active species. Inorganic Chemistry . ISSN Print Edition ISSN: 0020-1669 Web Edition ISSN: 1520-510X
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  seek permission
• Type:
  other