Description

• Title:
  Analysis of the quadruple lsu mutant reveals molecular determinants of the role of LSU proteins in sulfur assimilation in Arabidopsis
• Creator:
  Piotrowska, Justyna;
  Wawrzyńska, Anna;
  Olszak, Marcin;
  Krzyszton, Michal;
  Apodiakou, Anastasia;
  Alseekh, Saleh;
  López Ramos, Jose Maria;
  Hoefgen, Rainer;
  Kopriva, Stanislav;
  Sirko, Agnieszka
• Description:
  Because plants are immobile, they have developed intricate mechanisms to sense and absorb nutrients, adjusting their growth and development accordingly. Sulfur is an essential macroelement, but our understanding of its metabolism and homeostasis is limited. LSU (RESPONSE TO LOW SULFUR) proteins are plant-specific proteins with unknown molecular functions and were first identified during transcriptomic studies on sulfur deficiency in Arabidopsis. These proteins are crucial hubs that integrate environmental signals and are involved in the response to various stressors. Herein, we report the direct involvement of LSU proteins in primary sulfur metabolism. Our findings revealed that the quadruple lsu mutant, q-lsu-KO, which was grown under nonlimiting sulfate conditions, exhibited a molecular response resembling that of sulfur-deficient wild-type plants. This led us to explore the interactions of LSU proteins with sulfate reduction pathway enzymes. We found that all LSU proteins interact with ATPS1 and ATPS3 isoforms of ATP sulfurylase, all three isoforms of adenosine 5´phosphosulfate reductase (APR), and sulfite reductase (SiR). Additionally, in vitro assays revealed that LSU1 enhances the enzymatic activity of SiR. These results highlight the supportive role of LSU proteins in the sulfate reduction pathway.
• Object availability:
  free access
• Date:
  2024.11.29
• Type:
  Article;
  PeerReviewed
• Language:
  English
• Format:
  application/pdf
• Publisher:
  Wiley Online Library
• Relation: http://eprints.ibb.waw.pl/2491 ;https://onlinelibrary.wiley.com/journal/1365313x ;doi: 10.1111/tpj.17155
• Subject:
  Q Science (General)
• Identifier: http://eprints.ibb.waw.pl/2491/1/Piotrowska-PlantJournal.pdf ;
  Piotrowska, Justyna and Wawrzyńska, Anna and Olszak, Marcin and Krzyszton, Michal and Apodiakou, Anastasia and Alseekh, Saleh and López Ramos, Jose Maria and Hoefgen, Rainer and Kopriva, Stanislav and Sirko, Agnieszka (2024) Analysis of the quadruple lsu mutant reveals molecular determinants of the role of LSU proteins in sulfur assimilation in Arabidopsis. A non-canonical function of Arabidopsis ERECTA proteinsand a role of the SWI3B subunit of the SWI/SNF chromatinremodeling complex in gibberellin signalingElzbieta . Polska. ISSN 1365-313X
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  seek permission
• Type:
  other