Object's details: Structural Model of the Bilitranslocase Transmembrane Domain Supported by NMR and FRET Data.

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Structural Model of the Bilitranslocase Transmembrane Domain Supported by NMR and FRET Data.
Creator:
Choudhury, Amrita Roy;
Sikorska, Emilia;
van den Boom, Johannes;
Bayer, Peter;
Popenda, Łukasz;
Szutkowski, Kosma;
Jurga, Stefan;
Bonomi, Massimiliano;
Sali, Andrej;
Zhukov, Igor;
Passamonti, Sabina;
Novič, Marjana
Description:
We present a 3D model of the four transmembrane (TM) helical regions of bilitranslocase (BTL), a structurally uncharacterized protein that transports organic anions across the cell membrane. The model was computed by considering helix-helix interactions as primary constraints, using Monte Carlo simulations. The interactions between the TM2 and TM3 segments have been confirmed by Förster resonance energy transfer (FRET) spectroscopy and nuclear magnetic resonance (NMR) spectroscopy, increasing our confidence in the model. Several insights into the BTL transport mechanism were obtained by analyzing the model. For example, the observed cis-trans Leu-Pro peptide bond isomerization in the TM3 fragment may indicate a key conformational change during anion transport by BTL. Our structural model of BTL may facilitate further studies, including drug discovery.
Object availability:
free access
Date:
2015
Type:
Article;
PeerReviewed
Language:
English
Format:
application/pdf
Relation: http://eprints.ibb.waw.pl/1001
Subject:
QD Chemistry
Identifier: http://eprints.ibb.waw.pl/1001/1/plos.one_2015_10_0135455.pdf ;
Choudhury, Amrita Roy and Sikorska, Emilia and van den Boom, Johannes and Bayer, Peter and Popenda, Łukasz and Szutkowski, Kosma and Jurga, Stefan and Bonomi, Massimiliano and Sali, Andrej and Zhukov, Igor and Passamonti, Sabina and Novič, Marjana (2015) Structural Model of the Bilitranslocase Transmembrane Domain Supported by NMR and FRET Data. PloS one, 10 (8). e0135455. ISSN 1932-6203
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: Structural Model of the Bilitranslocase Transmembrane Domain Supported by NMR and FRET Data.

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