Object's details: Mutagenic potency of MMS-induced 1meA/3meC lesions in E. coli.

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Mutagenic potency of MMS-induced 1meA/3meC lesions in E. coli.
Creator:
Nieminuszczy, Jadwiga;
Mielecki, Damian;
Sikora, Anna;
Wrzesiński, Michał;
Chojnacka, Aleksandra;
Krwawicz, Joanna;
Janion, Celina;
Grzesiuk, Elzbieta
Description:
The mutagenic activity of MMS in E. coli depends on the susceptibility of DNA bases to methylation and their repair by cellular defense systems. Among the lesions in methylated DNA is 1meA/3meC, which is recently recognized as being mutagenic. In this report, special attention is focused on the mutagenic properties of 1meA/3meC which, by the activity of AlkB-dioxygenase, are quickly and efficiently converted to natural A/C bases in the DNA of E. coli alkB(+) strains, preventing 1meA/3meC-induced mutations. We have found that in the absence of AlkB-mediated repair, MMS treatment results in an increased frequency of four types of base substitutions: GC-->CG, GC-->TA, AT-->CG, and AT-->TA, whereas overproduction of PolV in CC101-106 alkB(-)/pRW134 strains leads to a markedly elevated level of GC-->TA, GC-->CG, and AT-->TA transversions. It has been observed that in the case of AB1157 alkB(-) strains, the MMS-induced and 1meA/3meC-dependent argE3-->Arg(+) reversion occurs efficiently, whereas lacZ(-)--> Lac(+) reversion in a set of CC101-106 alkB(-) strains occurs with much lower frequency. We considered several reasons for this discrepancy, namely, the possible variance in the level of the PolV activity, the effect of the PolIV contents that is higher in CC101-106 than in AB1157 strains and the different genetic cell backgrounds in CC101-106 alkB(-) and AB1157 alkB(-) strains, respectively. We postulate that the difference in the number of targets undergoing mutation and different reactivity of MMS with ssDNA and dsDNA are responsible for the high (argE3-->Arg(+)) and low (lacZ(-) --> Lac(+)) frequency of MMS-induced mutations.
Object availability:
free access
Date:
2009
Type:
Article;
PeerReviewed
Language:
English
Format:
application/pdf
Publisher:
Wiley-Blackwell
Relation: http://eprints.ibb.waw.pl/270 ;http://www.ems-us.org/content/publications/journal.asp
Subject:
Q Science (General);
QH426 Genetics
Identifier: http://eprints.ibb.waw.pl/270/2/2009_TLS_Grzesiuk.pdf ;
Nieminuszczy, Jadwiga and Mielecki, Damian and Sikora, Anna and Wrzesiński, Michał and Chojnacka, Aleksandra and Krwawicz, Joanna and Janion, Celina and Grzesiuk, Elzbieta (2009) Mutagenic potency of MMS-induced 1meA/3meC lesions in E. coli. Environmental and Molecular Mutagenesis (EMM), 50 (9). pp. 791-9. ISSN 1098-2280
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: Mutagenic potency of MMS-induced 1meA/3meC lesions in E. coli.

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