Description

• Title:
  Cytokine profiling in exhaled breath condensate after exercise challenge in asthmatic children with post-exercise symptoms
• Creator:
  Sielski, Piotr;
  Majak, Paweł;
  Jerzyńska, Joanna;
  Bojo, Magdalena;
  Brzozowska, Agnieszka;
  Koczkowska, Magdalena;
  Stelmach, Włodzimierz;
  Stelmach, Rafał;
  Janas, Anna;
  Stelmach, Iwona
• Contributor:
  Faculty of Mathematics and Computer Science, University of Lodz, Lodz, Poland;
  Department of Internal Medicine, Asthma and Allergy, Barlicki University Hospital, Medical University of Lodz, Lodz, Poland;
  Department of Paediatrics and Allergy, Medical University of Lodz, Lodz, Poland;
  Department of Biology and Genetics, Medical University of Gdansk, Gdansk, Poland;
  Department of Social and Preventive Medicine, Medical University of Lodz, Lodz, Poland;
  Institute of Dental Surgery, Faculty of Medicine and Dentistry, Medical University of Lodz, Lodz, Poland
• Description:
  Introduction: Markers of exhaled breath condensate (EBC) correlate with lung function impairment, airway remodeling and different aspects of the disease such as exercise-induced bronchoconstriction (EIB). Aim of the study was to determine the cytokine profile in EBC of children with asthma after an exercise treadmill challenge in order to obtain clinically useful information about mechanisms of EIB; also, to assess correlations between cytokine concentrations in EBC and clinical characteristics of the patients. Material and methods: The study population consisted of 25 randomly selected children, aged 8 to 19 years, with asthma and EIB symptoms despite the use of control medications. Patients on the day of the study visit underwent fractional exhaled nitric oxide measurement (FeNO) and baseline spirometry, performed an exercise treadmill challenge (ETC), and EBC samples were obtained at the end of the ETC. Results: In asthmatic children with positive ETC, monocyte hemotactic protein-1 (MCP-1) and IL-16 adjusted to pre-EBC forced expiratory volume in 1 s (FEV1) were significantly higher compared to children with negative ETC (p = 0.022 and p = 0.017 respectively). After adjustment to pre-EBC FEV1 other cytokines (IL-4, IL-5, IL-6, IL-8, MIG, TNF-) were not related to post-exercise changes in FEV1. Conclusions: We observed a specific inflammatory profile in the airways of asthmatic children with bronchoconstriction induced by exercise. The concentration of cytokines in EBC depended on the post-exercise decrease in FEV1, which was measured by the area under the curve (AUC). MCP-1 and IL-16, adjusted to pre-EBC FEV1, were significantly higher in children with a positive exercise challenge compared to those with a negative one.;
  This study was funded by grant 503-2056-1 from the Medical University of Lodz, Poland. ClinicalTrials.gov Identifier: NCT01798823. The study was approved by the Medical University of Lodz Ethics committee, Poland. Written consent from the patients and their parents was obtained.
• Object availability:
  free access
• Rights: http://creativecommons.org/licenses/by-nc-sa/4.0 ;
  Uznanie autorstwa-Użycie niekomercyjne-Na tych samych warunkach 4.0 Międzynarodowe
• Date:
  2021.10.15;
  2016
• Type:
  Article
• Language:
  English
• Format:
  application/pdf
• Publisher:
  Termedia Publishing House
• Relation:
  Archives of Medical Science;12;
  4
• Subject:
  asthma;
  children;
  cytokines;
  exhaled breath condensate;
  Exercise-induced bronchoconstriction;
  exercise-induced bronchoconstriction
• Identifier:
  Majak P, Jerzyńska J, Bojo M, et al. Cytokine profiling in exhaled breath condensate after exercise challenge in asthmatic children with post-exercise symptoms. Archives of Medical Science. 2016;12(4):778-784. doi:10.5114/aoms.2015.48547.;
  1734-1922;
  http://hdl.handle.net/11089/39389 ;
  1896-9151;
  10.5114/aoms.2015.48547
• Data provider:
  Repozytorium Uniwersytetu Łódzkiego
• Can I use it?:
  seek permission
• Type:
  image