Object's details: Gene and protein expression of O-GlcNAc-cycling enzymes in human laryngeal cancer

Provider:Repozytorium Uniwersytetu Łódzkiego

Description

Title:
Gene and protein expression of O-GlcNAc-cycling enzymes in human laryngeal cancer
Creator:
Starska, Katarzyna;
Forma, Ewa;
Brzezińska-Błaszczyk, Ewa;
Lewy-Trenda, Iwona;
Bryś, Magdalena;
Jóźwiak, Paweł;
Krześlak, Anna
Contributor:
University of Łódź, Department of Cytobiochemistry;
Medical University of Łódź;
katarzyna.starska@umed.lodz.pl
Description:
Aberrant protein O-GlcNAcylation may contribute to the development and malignant behavior of many cancers. This modification is controlled by O-linked β-N-acetylglucosamine transferase (OGT) and O-GlcNAcase (OGA). The aim of this study was to determine the expression of O-GlcNAc cycling enzymes mRNA/protein and to investigate their relationship with clinicopathological parameters in laryngeal cancer. The mRNA levels of OGT and MGEA5 genes were determined in 106 squamous cell laryngeal cancer (SCLC) cases and 73 non-cancerous adjacent laryngeal mucosa (NCLM) controls using quantitative real-time PCR. The level of OGT and OGA proteins was analyzed by Western blot. A positive expression of OGT and MGEA5 transcripts and OGT and OGA proteins was confirmed in 75.5 and 68.9 % and in 43.7 and 59.4 % samples of SCLC, respectively. Higher levels of mRNA/protein for both OGT and OGA as well as significant increases of 60 % in total protein O-GlcNAcylation levels were noted in SCLC compared with NCLM (p < 0.05). As a result, an increased level of OGT and MGEA5 mRNA was related to larger tumor size, nodal metastases, higher grade and tumor behavior according to TFG scale, as well as incidence of disease recurrence (p < 0.05). An inverse association between OGT and MGEA5 transcripts was determined with regard to prognosis (p < 0.05). In addition, the highest OGT and OGA protein levels were observed in poorly differentiated tumors (p < 0.05). No correlations with other parameters were noted, but the results showed a trend of more advanced tumors to be more frequently OGT and OGA positive. The results suggest that increased O-GlcNAcylation may have an effect on tumor aggressiveness and prognosis in laryngeal cancer.;
This work was supported, in part, by a grant from the National Science Council, Poland (N403 043 32/2326), by the statutory fund of the Department of Cytobiochemistry, University of Łódź, Poland (506/811)
Object availability:
free access
Rights:
Uznanie autorstwa 3.0 Polska;
http://creativecommons.org/licenses/by/3.0/pl
Date:
2016.04.05;
2014
Type:
Article
Language:
English
Format:
application/pdf
Publisher:
Springer International Publishing
Relation:
Clinical and Experimental Medicine;4;
15
Subject:
O-GlcNAc;
OGT;
OGA (MGEA5);
Tumor front grading (TFG);
Laryngeal cancer
Identifier: http://hdl.handle.net/11089/17685 ;
1591-9528;
10.1007/s10238-014-0318-1
Data provider:
Repozytorium Uniwersytetu Łódzkiego

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