Description

• Title:
  Genistein inhibits activities of methylenetetrahydrofolate reductase and lactate dehydrogenase, enzymes which use NADH as a substrate
• Creator:
  Grabowski, Michał;
  Banecki, Bogdan;
  Kadziński, Leszek;
  Jakóbkiewicz-Banecka, Joanna;
  Kaźmierkiewicz, Rajmund;
  Gabig-Cimińska, Magdalena;
  Węgrzyn, Grzegorz;
  Węgrzyn, Alicja;
  Banecka-Majkutewicz, Zyta
• Description:
  Genistein (5, 7-dihydroxy-3- (4-hydroxyphenyl)-4H-1 benzopyran-4-one) is a natural isoflavone revealing many biological activities. Thus, it is considered as a therapeutic compound in as various disorders as cancer, infections and genetic diseases. Here, we demonstrate for the first time that genistein inhibits activities of bacterial methylenetetrahydrofolate reductase (MetF) and lactate dehydrogenase(LDH). Both enzymes use NADH as a substrate, and results of biochemical as well as molecular modeling studies with MetF suggest that genistein may interfere with binding of this dinucleotide to the enzyme. These results have implications for our understanding of biological functions of genistein and its effects on cellular metabolism.
• Object availability:
  free access
• Date:
  2015
• Type:
  Article;
  PeerReviewed
• Language:
  English
• Format:
  application/pdf
• Publisher:
  Elsevier
• Relation: http://eprints.ibb.waw.pl/1133 ;http://www.sciencedirect.com/science/article/pii/S0006291X15303946
• Subject:
  Q Science (General);
  QH301 Biology;
  QH426 Genetics;
  R Medicine (General)
• Identifier: http://eprints.ibb.waw.pl/1133/1/1-s2.0-S0006291X15303946-main.pdf ;
  Grabowski, Michał and Banecki, Bogdan and Kadziński, Leszek and Jakóbkiewicz-Banecka, Joanna and Kaźmierkiewicz, Rajmund and Gabig-Cimińska, Magdalena and Węgrzyn, Grzegorz and Węgrzyn, Alicja and Banecka-Majkutewicz, Zyta (2015) Genistein inhibits activities of methylenetetrahydrofolate reductase and lactate dehydrogenase, enzymes which use NADH as a substrate. Biochemical and biophysical research communications, 465 (3). pp. 363-367. ISSN 0006-291X
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  seek permission
• Type:
  other