Object's details: Two Strategies of Pseudomonas syringae to Avoid Recognition of the HopQ1 Effector in Nicotiana Species

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Two Strategies of Pseudomonas syringae to Avoid Recognition of the HopQ1 Effector in Nicotiana Species
Creator:
Zembek, Patrycja;
Danilecka, Aleksandra;
Hoser, Rafał;
Eschen-Lippold, Lennart;
Benicka, Marta;
Grech-Baran, Marta;
Rymaszewski, Wojciech;
Barymow-Filoniuk, Izabela;
Morgiewicz, Karolina;
Kwiatkowski, Jakub;
Piechocki, Marcin;
Poznański, Jarosław;
Lee, Justin;
Hennig, Jacek;
Krzymowska, Magdalena
Description:
Pseudomonas syringae employs a battery of type three secretion effectors to subvert plant immune responses. In turn, plants have developed receptors that recognize some of the bacterial effectors. Two strain-specific HopQ1 effector variants (for Hrp outer protein Q) from the pathovars phaseolicola 1448A (Pph) and tomato DC3000 (Pto) showed considerable differences in their ability to evoke disease symptoms in Nicotiana benthamiana. Surprisingly, the variants differ by only six amino acids located mostly in the N-terminal disordered region of HopQ1. We found that the presence of serine 87 and leucine 91 renders PtoHopQ1 susceptible to N-terminal processing by plant proteases. Substitutions at these two positions did not strongly affect PtoHopQ1 virulence properties in a susceptible host but they reduced bacterial growth and accelerated onset of cell death in a resistant host, suggesting that N-terminal mutations rendered PtoHopQ1 susceptible to processing in planta and, thus, represent a mechanism of recognition avoidance. Furthermore, we found that co-expression of HopR1, another effector encoded within the same gene cluster masks HopQ1 recognition in a strain-dependent manner. Together, these data suggest that HopQ1 is under high host-pathogen co-evolutionary selection pressure and P. syringae may have evolved differential effector processing or masking as two independent strategies to evade HopQ1 recognition, thus revealing another level of complexity in plant – microbe interactions.
Object availability:
free access
Date:
2018
Type:
Article;
PeerReviewed
Language:
English
Format:
application/pdf
Relation: http://eprints.ibb.waw.pl/1617 ;http://doi.org/10.3389/fpls.2018.00978 ;doi:10.3389/fpls.2018.00978
Subject:
QR Microbiology
Identifier: http://eprints.ibb.waw.pl/1617/1/fpls-09-00978.pdf ;
Zembek, Patrycja and Danilecka, Aleksandra and Hoser, Rafał and Eschen-Lippold, Lennart and Benicka, Marta and Grech-Baran, Marta and Rymaszewski, Wojciech and Barymow-Filoniuk, Izabela and Morgiewicz, Karolina and Kwiatkowski, Jakub and Piechocki, Marcin and Poznański, Jarosław and Lee, Justin and Hennig, Jacek and Krzymowska, Magdalena (2018) Two Strategies of Pseudomonas syringae to Avoid Recognition of the HopQ1 Effector in Nicotiana Species. Frontiers in Plant Science, 9 . ISSN 1664-462X
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: Two Strategies of Pseudomonas syringae to Avoid Recognition of the HopQ1 Effector in Nicotiana Species

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