Description

• Title:
  Isolation and characterization of 8 microsatellite loci for the ‘‘killer shrimp’’, an invasive Ponto-Caspian amphipod Dikerogammarus villosus (Crustacea: Amphipoda)
• Creator:
  Rewicz, Tomasz;
  Wattier, Rémi A.;
  Rigaud, Thierry;
  Bacela-Spychalska, Karolina;
  Grabowski, Michal
• Contributor:
  Tomasz Rewicz - Department of Invertebrate Zoology and Hydrobiology, University of Lodz;
  Rémi A.Wattier - Equipe Ecologie Evolutive, Universite´ de Bourgogne;
  Thierry Rigaud - Equipe Ecologie Evolutive, Universite´ de Bourgogne;
  Karolina Bacela-Spychalska - Department of Invertebrate Zoology and Hydrobiology, University of Lodz;
  Michał Grabowski - Department of Invertebrate Zoology and Hydrobiology, University of Lodz
• Description:
  Dikerogammarus villosus is a freshwater amphipod of the Ponto-Caspian origin recognized as one of the 100 worst alien species in Europe, having negative impact on biodiversity and functioning of the invaded aquatic ecosystems. The species has a wide ecophysiological tolerance and during the last 20 years it has rapidly spread throughout European inland waters. In consequence, it presents a major conservation management problem. We describe eight polymorphic microsatellite loci developed for D. villosus by combining a biotin-enrichment protocol and new generation 454GS-FLX Titanium pyrosequencing technology. When genotyped in 64 individuals from two locations, the loci exhibited a mean diversity of 4.87 alleles per locus (2–13). The mean observed and expected heterozygosities were, respectively, 0.439 (0.091–0.844) and 0.468 (0.089–0.843). Gametic disequilibrium was not detected for any pair of loci. The microsatellite markers will be a valuable tool in assessing the demographic processes associated with invasion of the killer shrimp from a genetic point of view.
• Object availability:
  free access
• Rights:
  Uznanie autorstwa 3.0 Polska;
  http://creativecommons.org/licenses/by/3.0/pl
• Date:
  2015.03.31;
  2015
• Type:
  Article
• Language:
  English
• Format:
  application/pdf
• Publisher:
  Springer Science+Business Media B.V.
• Relation:
  Molecular Biology Reports;January 2015, Volume 42, Issue 1
• Subject:
  Invasive species;
  Population genetics;
  Dikerogammarus villosus;
  Biological invasions;
  Polymorphic loci
• Identifier:
  1573-4978;
  http://hdl.handle.net/11089/7580 ;
  10.1007/s11033-014-3742-0
• Data provider:
  Repozytorium Uniwersytetu Łódzkiego
• Can I use it?:
  check license
• Type:
  image