Description

• Title:
  Contribution of transcription-coupled DNA repair to MMS-induced mutagenesis in E. coli strains deficient in functional AlkB protein.
• Creator:
  Wrzesiński, Michał;
  Nieminuszczy, Jadwiga;
  Sikora, Anna;
  Mielecki, Damian;
  Chojnacka, Aleksandra;
  Kozłowski, Marek;
  Krwawicz, Joanna;
  Grzesiuk, Elzbieta
• Description:
  In Escherichia coli the alkylating agent methyl methanesulfonate (MMS) induces defense systems (adaptive and SOS responses), DNA repair pathways, and mutagenesis. We have previously found that AlkB protein induced as part of the adaptive (Ada) response protects cells from the genotoxic and mutagenic activity of MMS. AlkB is a non-heme iron (II), alpha-ketoglutarate-dependent dioxygenase that oxidatively demethylates 1meA and 3meC lesions in DNA, with recovery of A and C. Here, we studied the impact of transcription-coupled DNA repair (TCR) on MMS-induced mutagenesis in E. coli strain deficient in functional AlkB protein. Measuring the decline in the frequency of MMS-induced argE3-->Arg(+) revertants under transient amino acid starvation (conditions for TCR induction), we have found a less effective TCR in the BS87 (alkB(-)) strain in comparison with the AB1157 (alkB(+)) counterpart. Mutation in the mfd gene encoding the transcription-repair coupling factor Mfd, resulted in weaker TCR in MMS-treated and starved AB1157 mfd-1 cells in comparison to AB1157 mfd(+), and no repair in BS87 mfd(-) cells. Determination of specificity of Arg(+) revertants allowed to conclude that MMS-induced 1meA and 3meC lesions, unrepaired in bacteria deficient in AlkB, are the source of mutations. These include AT-->TA transversions by supL suppressor formation (1meA) and GC-->AT transitions by supB or supE(oc) formation (3meC). The repair of these lesions is partly Mfd-dependent in the AB1157 mfd-1 and totally Mfd-dependent in the BS87 mfd-1 strain. The nucleotide sequence of the mfd-1 allele shows that the mutated Mfd-1 protein, deprived of the C-terminal translocase domain, is unable to initiate TCR. It strongly enhances the SOS response in the alkB(-)mfd(-) bacteria but not in the alkB(+)mfd(-) counterpart.
• Object availability:
  free access
• Date:
  2010.06.01
• Type:
  Article;
  PeerReviewed
• Language:
  English
• Format:
  application/pdf
• Publisher:
  Elsevier
• Relation: http://eprints.ibb.waw.pl/44 ;http://linkinghub.elsevier.com/retrieve/pii/S0027-5107(10)00068-0 ;doi:10.1016/j.mrfmmm.2010.02.005
• Subject:
  QH301 Biology;
  QH426 Genetics
• Identifier: http://eprints.ibb.waw.pl/44/1/Mut_Res_Final.pdf ;
  Wrzesiński, Michał and Nieminuszczy, Jadwiga and Sikora, Anna and Mielecki, Damian and Chojnacka, Aleksandra and Kozłowski, Marek and Krwawicz, Joanna and Grzesiuk, Elzbieta (2010) Contribution of transcription-coupled DNA repair to MMS-induced mutagenesis in E. coli strains deficient in functional AlkB protein. Mutation research, 688 (1-2). pp. 19-27. ISSN 0027-5107
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  seek permission
• Type:
  other