Description

• Title:
  Bcr-Abl Amplification Plays a Major Role in Resistance to Tyrosine Kinase Inhibitors in K-562 Cell Line
• Creator:
  Czyżewski, Krzysztof;
  Skonieczka, Katarzyna;
  Różycki, Patryk;
  Kołodziej, Beata;
  Kuryło-Rafińska, Beata;
  Kubicka, Małgorzata;
  Matiakowska, Karolina;
  Mucha, Barbara;
  Haus, Olga;
  Wysocki, Mariusz;
  Styczyński, Jan
• Description:
  An emerging problem in patients with chronic myeloid leukemia (CML) is increasing resistance to tyrosine kinase inhibitors (TKIs). To determine genetic and cellular mechanisms involved in the development of resistance to TKIs, nine imatinib-resistant cell lines were derived from K- 562 cell line followed by testing of drug sensitivity, multidrug resistance proteins and cytogenetic studies. In imatinib-resistant cell lines cross-resistance to daunorubicin, etoposide and cytarabine were observed whereas sensitivity to dasatinib, nilotinib, cyclophpsphamide, bortezomib and busulfan was preserved. Treatment with imatinib decreased PGP and LRP expression, however it did not significantly influence MRP1 expression. Amount of signals in FISH analysis from ABL, BCR and from fusion genes (BCR-ABL or ABL-BCR) was mostly higher in imatinib-resistant cell lines in comparison to parental K-562 cell line. We concluded that BCR-ABL amplification but not cellular sensitivity is the major mechanisms of resistance in K-562 cell line.
• Object availability:
  free access
• Rights:
  Prawa autorskie (c) 2014 Medical and Biological Sciences
• Date:
  2012.06.24
• Type:
  info:eu-repo/semantics/article;
  info:eu-repo/semantics/publishedVersion
• Language:
  English
• Source:
  Medical and Biological Sciences; Vol. 26 No. 3 (2012); 45-51;
  Medical and Biological Sciences; Tom 26 Nr 3 (2012); 45-51;
  1734-591X
• Format:
  application/pdf
• Publisher:
  Uniwersytet Mikołaja Kopernika w Toruniu
• Relation: https://apcz.umk.pl/MBS/article/view/v10251-012-0054-2/4368
• Subject:
  BCR-ABL amplification;
  chronic myeloid leukemia;
  drug resistance;
  imatinib;
  tyrosine kinase inhibitors;
  K-562 cell line;
  amplifikacja BCR-ABL;
  przewlekła białaczka szpikowa;
  oporność na cytostatyki;
  imatynib;
  inhibitory kinazy tyrozynowej;
  linia K-562
• Identifier: https://apcz.umk.pl/MBS/article/view/v10251-012-0054-2
• Data provider:
  Akademicka Platforma Czasopism
• Can I use it?:
  seek permission
• Type:
  text